RRC ID |
36474
|
著者 |
Nonomura K, Morohoshi A, Nakano M, Eiguchi M, Miyao A, Hirochika H, Kurata N.
|
タイトル |
A germ cell specific gene of the ARGONAUTE family is essential for the progression of premeiotic mitosis and meiosis during sporogenesis in rice.
|
ジャーナル |
Plant Cell
|
Abstract |
The rice (Oryza sativa) genome contains 18 copies of genes of the ARGONAUTE (AGO) family. Although AGO members play important roles in RNA-mediated silencing during plant development, a family member that is specifically involved in sexual reproduction has not been identified in plants. We identified the rice AGO gene MEIOSIS ARRESTED AT LEPTOTENE1 (MEL1) from the analysis of seed-sterile mutants. In the mel1 mutant, chromosome condensation was arrested at early meiotic stages and irregularly sized, multinucleated, and vacuolated pollen mother cells (PMCs) frequently appeared in developing anthers. In addition, histone H3 lysine-9 dimethylation of pericentromeres was rarely reduced and modification of the nucleolar-organizing region was altered in mel1 mutant PMCs. The mutation also affected female germ cell development. These results indicate that the germ cell-specific rice MEL1 gene regulates the cell division of premeiotic germ cells, the proper modification of meiotic chromosomes, and the faithful progression of meiosis, probably via small RNA-mediated gene silencing, but not the initiation and establishment of germ cells themselves.
|
巻・号 |
19(8)
|
ページ |
2583-94
|
公開日 |
2007-8-1
|
DOI |
10.1105/tpc.107.053199
|
PII |
tpc.107.053199
|
PMID |
17675402
|
PMC |
PMC2002623
|
MeSH |
Amino Acid Sequence
Arabidopsis Proteins / metabolism*
Argonaute Proteins
Chromatin / metabolism
Chromosomes, Plant / metabolism
Gametogenesis*
Gene Expression Profiling
Gene Expression Regulation, Plant
Genes, Plant
Germ Cells / metabolism*
Meiosis*
Meiotic Prophase I
Mitosis*
Models, Biological
Molecular Sequence Data
Mutation / genetics
Oryza / cytology*
Oryza / genetics
Phylogeny
Plant Proteins / chemistry
Plant Proteins / genetics*
Plant Proteins / metabolism
Pollen / cytology
Pollen / ultrastructure
RNA, Messenger / genetics
RNA, Messenger / metabolism
|
IF |
9.618
|
引用数 |
220
|
WOS 分野
|
PLANT SCIENCES
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
リソース情報 |
イネ |
Sterile line (STER-NIG0009) (MEL1) |