| RRC ID |
36502
|
| 著者 |
Robellet X, Thattikota Y, Wang F, Wee TL, Pascariu M, Shankar S, Bonneil É, Brown CM, D'Amours D.
|
| タイトル |
A high-sensitivity phospho-switch triggered by Cdk1 governs chromosome morphogenesis during cell division.
|
| ジャーナル |
Genes Dev
|
| Abstract |
The initiation of chromosome morphogenesis marks the beginning of mitosis in all eukaryotic cells. Although many effectors of chromatin compaction have been reported, the nature and design of the essential trigger for global chromosome assembly remain unknown. Here we reveal the identity of the core mechanism responsible for chromosome morphogenesis in early mitosis. We show that the unique sensitivity of the chromosome condensation machinery for the kinase activity of Cdk1 acts as a major driving force for the compaction of chromatin at mitotic entry. This sensitivity is imparted by multisite phosphorylation of a conserved chromatin-binding sensor, the Smc4 protein. The multisite phosphorylation of this sensor integrates the activation state of Cdk1 with the dynamic binding of the condensation machinery to chromatin. Abrogation of this event leads to chromosome segregation defects and lethality, while moderate reduction reveals the existence of a novel chromatin transition state specific to mitosis, the intertwist configuration. Collectively, our results identify the mechanistic basis governing chromosome morphogenesis in early mitosis and how distinct chromatin compaction states can be established via specific thresholds of Cdk1 kinase activity.
|
| 巻・号 |
29(4)
|
| ページ |
426-39
|
| 公開日 |
2015-2-15
|
| DOI |
10.1101/gad.253294.114
|
| PII |
29/4/426
|
| PMID |
25691469
|
| PMC |
PMC4335297
|
| MeSH |
Adenosine Triphosphatases / chemistry
Adenosine Triphosphatases / metabolism
Cell Division / genetics*
Chromatin Assembly and Disassembly / genetics
Chromosomal Proteins, Non-Histone / metabolism
Chromosomes, Fungal / genetics*
Cyclin-Dependent Kinases / metabolism*
DNA-Binding Proteins / chemistry
DNA-Binding Proteins / metabolism
Genes, Switch / physiology
Mitosis
Multiprotein Complexes / chemistry
Multiprotein Complexes / metabolism
Phosphorylation
Protein Binding
Saccharomyces cerevisiae / cytology*
Saccharomyces cerevisiae / genetics*
Saccharomyces cerevisiae Proteins / metabolism
|
| IF |
9.527
|
| 引用数 |
26
|
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
GENETICS & HEREDITY
CELL BIOLOGY
|
| リソース情報 |
| 酵母 |
YNK54 - BY25598 |
