| RRC ID |
3813
|
| 著者 |
Morita Y, Matsuyama H, Serizawa A, Takeya T, Kawakami H.
|
| タイトル |
Identification of angiogenin as the osteoclastic bone resorption-inhibitory factor in bovine milk.
|
| ジャーナル |
Bone
|
| Abstract |
We identified, for the first time, the factor responsible for inhibiting osteoclast-mediated bone resorption in the basic protein fraction of bovine milk (milk basic protein, MBP). The protein was purified by a combination of ion and gel column chromatography from MBP, based on its activity to prevent unfractionated rabbit bone cells from forming pits on dentine slices. It was found to have a molecular weight of 15 kDa on SDS-PAGE, and the sequence of the N-terminal 25 amino acid residues was identical to that of bovine angiogenin. The purified bovine angiogenin inhibited the pit-forming activity of both unfractionated bone cells and purified osteoclasts in a dose-dependent manner, and the inhibitory activity was markedly suppressed by treatment with anti-bovine angiogenin antibody. The inhibitory activity was confirmed in mice both in vitro and in vivo. Treatment of osteoclasts with bovine angiogenin resulted in an impairment of the formation F-actin ring and a reduction in the mRNA levels of TRAP and cathepsin K, both known to be essential for the bone resorption activity of osteoclasts. These results suggest that bovine angiogenin is the substance mainly responsible for the inhibitory effect of bovine milk on osteoclast-mediated bone resorption, and that it exerts its activity by acting directly on the osteoclasts.
|
| 巻・号 |
42(2)
|
| ページ |
380-7
|
| 公開日 |
2008-2-1
|
| DOI |
10.1016/j.bone.2007.10.012
|
| PII |
S8756-3282(07)00777-6
|
| PMID |
18055286
|
| MeSH |
Animals
Base Sequence
Bone Resorption / metabolism
Bone Resorption / pathology*
Conserved Sequence
Female
Gene Expression Regulation / drug effects
Mice
Microscopy, Electron, Scanning
Milk / chemistry*
Milk Proteins / chemistry
Milk Proteins / isolation & purification
Milk Proteins / pharmacology
Molecular Sequence Data
Osteoclasts / drug effects*
Osteoclasts / metabolism
Ribonuclease, Pancreatic / chemistry
Ribonuclease, Pancreatic / isolation & purification
Ribonuclease, Pancreatic / pharmacology*
Sequence Alignment
|
| IF |
4.147
|
| 引用数 |
22
|
|
WOS 分野
|
ENDOCRINOLOGY & METABOLISM
|
| リソース情報 |
| 遺伝子材料 |
pCAhMCSF (RDB01524) |
