RRC ID 3961
著者 Zhao J, Niu W, Yao J, Mohr S, Marcotte EM, Lambowitz AM.
タイトル Group II intron protein localization and insertion sites are affected by polyphosphate.
ジャーナル PLoS Biol
Abstract Mobile group II introns consist of a catalytic intron RNA and an intron-encoded protein with reverse transcriptase activity, which act together in a ribonucleoprotein particle to promote DNA integration during intron mobility. Previously, we found that the Lactococcus lactis Ll.LtrB intron-encoded protein (LtrA) expressed alone or with the intron RNA to form ribonucleoprotein particles localizes to bacterial cellular poles, potentially accounting for the intron's preferential insertion in the oriC and ter regions of the Escherichia coli chromosome. Here, by using cell microarrays and automated fluorescence microscopy to screen a transposon-insertion library, we identified five E. coli genes (gppA, uhpT, wcaK, ynbC, and zntR) whose disruption results in both an increased proportion of cells with more diffuse LtrA localization and a more uniform genomic distribution of Ll.LtrB-insertion sites. Surprisingly, we find that a common factor affecting LtrA localization in these and other disruptants is the accumulation of intracellular polyphosphate, which appears to bind LtrA and other basic proteins and delocalize them away from the poles. Our findings show that the intracellular localization of a group II intron-encoded protein is a major determinant of insertion-site preference. More generally, our results suggest that polyphosphate accumulation may provide a means of localizing proteins to different sites of action during cellular stress or entry into stationary phase, with potentially wide physiological consequences.
巻・号 6(6)
ページ e150
公開日 2008-6-24
DOI 10.1371/journal.pbio.0060150
PII 07-PLBI-RA-4042
PMID 18593213
PMC PMC2435150
MeSH Bacterial Proteins / analysis* Bacterial Proteins / genetics* Bacterial Proteins / metabolism DNA Transposable Elements / genetics* Escherichia coli / metabolism Introns* Microarray Analysis Polyphosphates / metabolism* RNA-Directed DNA Polymerase / analysis* RNA-Directed DNA Polymerase / genetics RNA-Directed DNA Polymerase / metabolism
IF 7.076
引用数 14
WOS 分野 BIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) CV-35(HMS174(DE3))