RRC ID 41317
著者 Ishii M, Suda Y, Kurokawa K, Nakano A.
タイトル COPI is essential for Golgi cisternal maturation and dynamics.
ジャーナル J Cell Sci
Abstract Proteins synthesized in the endoplasmic reticulum (ER) are transported to the Golgi and then sorted to their destinations. For their passage through the Golgi, one widely accepted mechanism is cisternal maturation. Cisternal maturation is fulfilled by the retrograde transport of Golgi-resident proteins from later to earlier cisternae, and candidate carriers for this retrograde transport are coat protein complex I (COPI)-coated vesicles. We examined the COPI function in cisternal maturation directly by 4D observation of the transmembrane Golgi-resident proteins in living yeast cells. COPI temperature-sensitive mutants and induced degradation of COPI proteins were used to knockdown COPI function. For both methods, inactivation of COPI subunits Ret1 and Sec21 markedly impaired the transition from cis to medial and to trans cisternae. Furthermore, the movement of cisternae within the cytoplasm was severely restricted when COPI subunits were depleted. Our results demonstrate the essential roles of COPI proteins in retrograde trafficking of the Golgi-resident proteins and dynamics of the Golgi cisternae.
巻・号 129(17)
ページ 3251-61
公開日 2016-9-1
DOI 10.1242/jcs.193367
PII jcs.193367
PMID 27445311
PMC PMC5047698
MeSH Coat Protein Complex I / metabolism* Golgi Apparatus / drug effects Golgi Apparatus / metabolism* Indoleacetic Acids / pharmacology Membrane Proteins / metabolism Mutation / genetics Proteolysis / drug effects Saccharomyces cerevisiae / drug effects Saccharomyces cerevisiae / metabolism* Temperature
IF 4.573
WOS 分野 CELL BIOLOGY
リソース情報
酵母 BYP6742 (pMK76) BYP6740 (pMK43)