RRC ID 4158
著者 Okazaki M, Higuchi K, Hanawa Y, Shiraiwa Y, Ezura H.
タイトル Cloning and characterization of a Chlamydomonas reinhardtii cDNA arylalkylamine N-acetyltransferase and its use in the genetic engineering of melatonin content in the Micro-Tom tomato.
ジャーナル J Pineal Res
Abstract Melatonin is found in a wide variety of plant species. Several investigators have studied the physiological roles of melatonin in plants. However, its role is not well understood because of the limited information on its biosynthetic pathway. To clarify melatonin biosynthesis in plants, we isolated a cDNA-coded arylalkylamine N-acetyltransferase (AANAT), a possible limiting enzyme for melatonin biosynthesis, from Chlamydomonas reinhardtii (designated as CrAANAT). The predicted amino acid sequence of CrAANAT shares 39.0% homology to AANAT from Ostreococcus tauri and lacks cAMP-dependent protein kinase phosphorylation sites in the N- and C-terminal regions that are conserved in vertebrates. The enzyme activity of CrAANAT was confirmed by in vitro assay using Escherichia coli. Transgenic plants constitutively expressing the CrAANAT were produced using Micro-Tom, a model cultivar of tomato (Solanum lycopersicum L.). The transgenic Micro-Tom exhibited higher melatonin content compared with wild type, suggesting that melatonin was synthesized from serotonin via N-acetylserotonin in plants. Moreover, the melatonin-rich transgenic Micro-Tom can be used to elucidate the role of melatonin in plant development.
巻・号 46(4)
ページ 373-82
公開日 2009-5-1
DOI 10.1111/j.1600-079X.2009.00673.x
PII JPI673
PMID 19552760
MeSH Amino Acid Sequence Animals Arylalkylamine N-Acetyltransferase / biosynthesis Arylalkylamine N-Acetyltransferase / genetics* Arylalkylamine N-Acetyltransferase / metabolism* Chlamydomonas reinhardtii / enzymology* Chlamydomonas reinhardtii / genetics Cloning, Molecular Data Interpretation, Statistical Genetic Engineering / methods Humans Kinetics Lycopersicon esculentum / genetics Lycopersicon esculentum / metabolism* Melatonin / biosynthesis* Melatonin / metabolism Molecular Sequence Data Phylogeny Plant Leaves / chemistry Plants, Genetically Modified / genetics Plants, Genetically Modified / metabolism* Recombinant Proteins / biosynthesis Recombinant Proteins / genetics Sequence Alignment
IF 14.528
引用数 55
WOS 分野 PHYSIOLOGY ENDOCRINOLOGY & METABOLISM NEUROSCIENCES
リソース情報
トマト TOMJPF00001