RRC ID 4490
著者 Hermansyah, Sugiyama M, Kaneko Y, Harashima S.
タイトル Yeast protein phosphatases Ptp2p and Msg5p are involved in G1-S transition, CLN2 transcription, and vacuole morphogenesis.
ジャーナル Arch Microbiol
Abstract We previously reported that double disruption of protein phosphatase (PPase) genes PTP2 (phosphotyrosine-specific PPase) and MSG5 (phosphotyrosine and phosphothreonine/serine-PPase) causes Ca(2+) sensitive growth, whereas the single disruptions do not. This finding suggests that Ptp2p and Msg5p are involved in Ca(2+)-induced stress response in a redundant manner. To gain insight into the molecular mechanism causing calcium sensitivity of the ptp2 msg5 double disruptant, we performed fluorescence-activated cell sorting analysis and found a delayed G1 phase. This delayed G1 was consistent with the defect in bud emergence, and reduced CLN2 transcription upon addition of CaCl(2). We also found that Slt2p is hyper-phosphorylated in the Deltaptp2 Deltamsg5 double disruptant and that the vacuole of the Deltaptp2 Deltamsg5 double disruptant is fragmented even in the absence of Ca(2+). These findings suggest that both Ptp2p and Msg5p are involved in the G1 to S transition and vacuole morphogenesis possibly through their regulation of Slt2 pathway.
巻・号 191(9)
ページ 721-33
公開日 2009-9-1
DOI 10.1007/s00203-009-0498-3
PMID 19680630
MeSH Calcium Chloride / metabolism Cell Cycle Cyclins / biosynthesis* Gene Deletion Phosphoric Monoester Hydrolases / genetics Phosphoric Monoester Hydrolases / metabolism* Protein Tyrosine Phosphatases / genetics Protein Tyrosine Phosphatases / metabolism* Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / growth & development Saccharomyces cerevisiae / physiology* Saccharomyces cerevisiae Proteins / biosynthesis* Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism* Transcription, Genetic Vacuoles / physiology*
IF 1.884
引用数 11
WOS 分野 MICROBIOLOGY
リソース情報
酵母 BY5209 (= SH5209) BY5210 (= SH5210)