| RRC ID |
4713
|
| 著者 |
Futahashi R, Sato J, Meng Y, Okamoto S, Daimon T, Yamamoto K, Suetsugu Y, Narukawa J, Takahashi H, Banno Y, Katsuma S, Shimada T, Mita K, Fujiwara H.
|
| タイトル |
yellow and ebony are the responsible genes for the larval color mutants of the silkworm Bombyx mori.
|
| ジャーナル |
Genetics
|
| Abstract |
Many larval color mutants have been obtained in the silkworm Bombyx mori. Mapping of melanin-synthesis genes on the Bombyx linkage map revealed that yellow and ebony genes were located near the chocolate (ch) and sooty (so) loci, respectively. In the ch mutants, body color of neonate larvae and the body markings of elder instar larvae are reddish brown instead of normal black. Mutations at the so locus produce smoky larvae and black pupae. F(2) linkage analyses showed that sequence polymorphisms of yellow and ebony genes perfectly cosegregated with the ch and so mutant phenotypes, respectively. Both yellow and ebony were expressed in the epidermis during the molting period when cuticular pigmentation occurred. The spatial expression pattern of yellow transcripts coincided with the larval black markings. In the ch mutants, nonsense mutations of the yellow gene were detected, whereas large deletions of the ebony ORF were detected in the so mutants. These results indicate that yellow and ebony are the responsible genes for the ch and so loci, respectively. Our findings suggest that Yellow promotes melanization, whereas Ebony inhibits melanization in Lepidoptera and that melanin-synthesis enzymes play a critical role in the lepidopteran larval color pattern.
|
| 巻・号 |
180(4)
|
| ページ |
1995-2005
|
| 公開日 |
2008-12-1
|
| DOI |
10.1534/genetics.108.096388
|
| PII |
genetics.108.096388
|
| 解説 |
ch(赤蟻）の原因遺伝子はショウジヨウバエのyellowのカイコホモログである。l11系統(ch/ch)では、カイコのyellowのエクソン４内部に14bpの欠失が存在する。K12系統（ch/ch)では、第３イントロンの先頭配列のGTがATに変異している影響でイントロン３が正しくスプライシングされない。
so(煤色)の原因遺伝子はショウジョウバエのebonyのカイコホモログである。i10系統とi41系統のso個体では、カイコのebonyのエクソン１４～16を含む4.7 kbの領域が欠失している。
|
| PMID |
18854583
|
| PMC |
PMC2600937
|
| MeSH |
Animals
Bombyx / genetics*
Bombyx / growth & development
Bombyx / metabolism
Genes, Insect*
Genetic Linkage
Insect Proteins / genetics
Larva / genetics
Larva / metabolism
Melanins / biosynthesis
Models, Genetic
Mutation
Phylogeny
Pigmentation / genetics*
|
| IF |
4.015
|
| 引用数 |
75
|
|
WOS 分野
|
GENETICS & HEREDITY
|
| リソース情報 |
| カイコ |
k12
l11
i10
i41 |
