RRC ID 47300
著者 Yeasmin AM, Waliullah TM, Kondo A, Kaneko A, Koike N, Ushimaru T.
タイトル Orchestrated Action of PP2A Antagonizes Atg13 Phosphorylation and Promotes Autophagy after the Inactivation of TORC1.
ジャーナル PLoS One
Abstract Target of rapamycin complex 1 (TORC1) phosphorylates autophagy-related Atg13 and represses autophagy under nutrient-rich conditions. However, when TORC1 becomes inactive upon nutrient depletion or treatment with the TORC1 inhibitor rapamycin, Atg13 dephosphorylation occurs rapidly, and autophagy is induced. At present, the phosphatases involved in Atg13 dephosphorylation remain unknown. Here, we show that two protein phosphatase 2A (PP2A) phosphatases, PP2A-Cdc55 and PP2A-Rts1, which are activated by inactivation of TORC1, are required for sufficient Atg13 dephosphorylation and autophagy induction after TORC1 inactivation in budding yeast. After rapamycin treatment, dephosphorylation of Atg13, activation of Atg1 kinase, pre-autophagosomal structure (PAS) formation and autophagy induction are all impaired in PP2A-deleted cells. Conversely, overexpression of non-phosphorylatable Atg13 suppressed defects in autophagy in PP2A mutant. This study revealed that the orchestrated action of PP2A antagonizes Atg13 phosphorylation and promotes autophagy after the inactivation of TORC1.
巻・号 11(12)
ページ e0166636
公開日 2016-1-1
DOI 10.1371/journal.pone.0166636
PII PONE-D-16-17492
PMID 27973551
PMC PMC5156417
MeSH Adaptor Proteins, Signal Transducing / metabolism* Autophagosomes Autophagy* Autophagy-Related Proteins / metabolism* Cell Cycle Proteins / metabolism* Cytoplasm / metabolism Green Fluorescent Proteins / metabolism Phosphorylation Protein Kinases / metabolism Protein Phosphatase 2 / metabolism* Saccharomyces cerevisiae / metabolism* Saccharomyces cerevisiae Proteins / metabolism* Sirolimus / chemistry Transcription Factors / metabolism*
IF 2.74
引用数 15
WOS 分野 CELL BIOLOGY
リソース情報
酵母 BY9943 (SCU2422)