RRC ID 48111
著者 Kishikawa S, Murata T, Kimura H, Shiota K, Yokoyama KK.
タイトル Regulation of transcription of the Dnmt1 gene by Sp1 and Sp3 zinc finger proteins.
ジャーナル Eur J Biochem
Abstract The Sp family is a family of transcription factors that bind to cis-elements in the promoter regions of various genes. Regulation of transcription by Sp proteins is based on interactions between a GC-rich binding site (GGGCGG) in DNA and C-terminal zinc finger motifs in the proteins. In this study, we characterized the GC-rich promoter of the gene for the DNA methyltransferase (Dnmt1) that is responsible for methylation of cytosine residues in mammals and plays a role in gene silencing. We found that a cis-element (nucleotides -161 to -147) was essential for the expression of the mouse gene for Dnmt1. DNA-binding assays indicated that transcription factors Sp1 and Sp3 bound to the same cis-element in this region in a dose-dependent manner. In Drosophila SL2 cells, which lack the Sp family of transcription factors, forced expression of Sp1 or Sp3 enhanced transcription from the Dnmt1 promoter. Stimulation by Sp1 and Sp3 were independent phenomena. Furthermore, cotransfection reporter assays with a p300-expression plasmid revealed the activation of the promoter of the Dnmt1 gene in the presence of Sp3. The transcriptional coactivator p300 interacted with Sp3 in vivo and in vitro. Our results indicate that expression of the Dnmt1 gene is controled by Sp1 and Sp3 and that p300 is involved in the activation by Sp3.
巻・号 269(12)
ページ 2961-70
公開日 2002-6-1
DOI 10.1046/j.1432-1033.2002.02972.x
PII 2972
PMID 12071960
MeSH 3T3 Cells Adenine / metabolism Animals Binding Sites Cells, Cultured DNA (Cytosine-5-)-Methyltransferase 1 DNA (Cytosine-5-)-Methyltransferases / genetics* DNA (Cytosine-5-)-Methyltransferases / metabolism DNA-Binding Proteins / metabolism DNA-Binding Proteins / physiology* Drosophila melanogaster E1A-Associated p300 Protein Genes, Regulator / genetics* Guanosine / metabolism Mice Nuclear Proteins / metabolism Nuclear Proteins / physiology Oligodeoxyribonucleotides / metabolism Promoter Regions, Genetic Sp1 Transcription Factor / metabolism Sp1 Transcription Factor / physiology* Sp3 Transcription Factor Trans-Activators / metabolism Trans-Activators / physiology Transcription Factors / metabolism Transcription Factors / physiology* Transcriptional Activation / physiology* Zinc Fingers / physiology
引用数 73
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
遺伝子材料 D1 (RDB3179) D2 (RDB3180) D4 (RDB3181).