RRC ID |
49565
|
著者 |
Narita H, Asaka Y, Ikura K, Matsumoto S, Sasaki R.
|
タイトル |
Isolation, characterization and expression of cationic peroxidase isozymes released into the medium of cultured tobacco cells.
|
ジャーナル |
Eur J Biochem
|
Abstract |
Three glycoproteins of 34, 38 and 40 kDa were isolated from the spent medium of suspension-cultured tobacco cells. The 38-kDa and 40-kDa proteins were highly cationic peroxidases with indistinguishable enzymic properties but their structural difference was confirmed by sequence analysis of the amino-terminal regions and the recognition specificity of monoclonal antibodies. The 34-kDa protein was a moderately cationic peroxidase with enzymic properties quite different from those of the 38-kDa and 40-kDa enzymes. They were undetectable in the spent medium during the cell-proliferation phase but became abundant in the medium during the cell-expansion phase. This was confirmed quantitatively with the 40-kDa protein using the 40-kDa-specific monoclonal antibody. The mRNA expression for 40-kDa protein was at a constant basal level in the cell-proliferation phase but increased in the cell-expansion phase.
|
巻・号 |
228(3)
|
ページ |
855-62
|
公開日 |
1995-3-15
|
PMID |
7737186
|
MeSH |
Amino Acid Sequence
Animals
Antibodies, Monoclonal / immunology
Blotting, Northern
Cells, Cultured
Culture Media
Enzyme-Linked Immunosorbent Assay
Female
Isoenzymes / genetics
Isoenzymes / immunology
Isoenzymes / isolation & purification*
Isoenzymes / metabolism
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Peroxidases / genetics
Peroxidases / immunology
Peroxidases / isolation & purification*
Peroxidases / metabolism
Plant Proteins / isolation & purification
Plant Proteins / metabolism
Plants, Toxic*
Tobacco / enzymology*
|
引用数 |
23
|
リソース情報 |
遺伝子材料 |
pC (RDB01851)
pD (RDB01852). |