RRC ID 4960
著者 Fukiya S, Arata M, Kawashima H, Yoshida D, Kaneko M, Minamida K, Watanabe J, Ogura Y, Uchida K, Itoh K, Wada M, Ito S, Yokota A.
タイトル Conversion of cholic acid and chenodeoxycholic acid into their 7-oxo derivatives by Bacteroides intestinalis AM-1 isolated from human feces.
ジャーナル FEMS Microbiol Lett
Abstract Secondary bile acid-producing bacteria were isolated from human feces to improve our appreciation of the functional diversity and redundancy of the intestinal microbiota. In total, 619 bacterial colonies were isolated using a nutrient-poor agar medium and the level of secondary bile acid formation was examined in each by a liquid culture, followed by thin-layer chromatography. Of five strains analyzed by 16S rRNA gene sequencing and biochemical testing, one was identified as Bacteroides intestinalis AM-1, which was not previously recognized as a secondary bile-acid producer. GC-MS revealed that B. intestinalis AM-1 converts cholic acid (CA) and chenodeoxycholic acid into their 7-oxo derivatives, 7-oxo-deoxycholic acid (7-oxo-DCA) and 7-oxo-lithocholic acid, respectively. Thus, B. intestinalis AM-1 possesses 7alpha-hydroxysteroid dehydrogenase (7alpha-HSDH) activity. In liquid culture, B. intestinalis AM-1 showed a relatively higher productivity of 7-oxo-DCA than Escherichia coli HB101 and Bacteroides fragilis JCM11019(T), which are known to possess 7alpha-HSDH activity. The level of 7alpha-HSDH activity was higher in B. intestinalis AM-1 than in the other two strains under the conditions tested. The 7alpha-HSDH activity in each of the three strains is not induced by CA; instead, it is regulated in a growth phase-dependent manner.
巻・号 293(2)
ページ 263-70
公開日 2009-4-1
DOI 10.1111/j.1574-6968.2009.01531.x
PII FML1531
PMID 19243441
MeSH Adult Bacterial Typing Techniques Bacteroides / classification Bacteroides / growth & development Bacteroides / isolation & purification Bacteroides / metabolism* Bacteroides fragilis / metabolism Biotransformation Chenodeoxycholic Acid / metabolism* Cholic Acid / metabolism* Chromatography, Thin Layer Culture Media / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Deoxycholic Acid / analogs & derivatives Deoxycholic Acid / metabolism Escherichia coli / metabolism Feces / microbiology* Female Humans Lithocholic Acid / analogs & derivatives Lithocholic Acid / metabolism Male Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Young Adult
IF 1.987
引用数 45
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(大腸菌) ME8568 (HB101)
一般微生物 JCM 11019 JCM 13265