| RRC ID |
4971
|
| 著者 |
Lee EG, Yoon SH, Das A, Lee SH, Li C, Kim JY, Choi MS, Oh DK, Kim SW.
|
| タイトル |
Directing vanillin production from ferulic acid by increased acetyl-CoA consumption in recombinant Escherichia coli.
|
| ジャーナル |
Biotechnol Bioeng
|
| Abstract |
The amplification of gltA gene encoding citrate synthase of TCA cycle was required for the efficient conversion of acetyl-CoA, generated during vanillin production from ferulic acid, to CoA, which is essential for vanillin production. Vanillin of 1.98 g/L was produced from the E. coli DH5alpha (pTAHEF-gltA) with gltA amplification in 48 h of culture at 3.0 g/L of ferulic acid, which was about twofold higher than the vanillin production of 0.91 g/L obtained by the E. coli DH5alpha (pTAHEF) without gltA amplification. The icdA gene encoding isocitrate dehydrogenase of TCA cycle was deleted to make the vanillin producing E. coli utilize glyoxylate bypass which enables more efficient conversion of acetyl-CoA to CoA in comparison with TCA cycle. The production of vanillin by the icdA null mutant of E. coli BW25113 harboring pTAHEF was enhanced by 2.6 times. The gltA amplification of the glyoxylate bypass in the icdA null mutant remarkably increased the production rate of vanillin with a little increase in the amount of vanillin production. The real synergistic effect of gltA amplification and icdA deletion was observed with use of XAD-2 resin reducing the toxicity of vanillin produced during culture. Vanillin of 5.14 g/L was produced in 24 h of the culture with molar conversion yield of 86.6%, which is the highest so far in vanillin production from ferulic acid using recombinant E. coli.
|
| 巻・号 |
102(1)
|
| ページ |
200-8
|
| 公開日 |
2009-1-1
|
| DOI |
10.1002/bit.22040
|
| PMID |
18683263
|
| MeSH |
Acetyl Coenzyme A / metabolism*
Adsorption
Benzaldehydes / metabolism*
Benzaldehydes / toxicity
Biotechnology / methods
Citrate (si)-Synthase / genetics
Citrate (si)-Synthase / metabolism
Coumaric Acids / metabolism*
Escherichia coli / genetics*
Escherichia coli / metabolism*
Escherichia coli Proteins / genetics
Escherichia coli Proteins / metabolism
Gene Deletion
Gene Dosage
Glyoxylates / metabolism
Isocitrate Dehydrogenase / genetics
Polystyrenes
|
| IF |
4.002
|
| 引用数 |
44
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| 原核生物(大腸菌) |
CV-25(DH5alpha)
ME9062(BW25113)
JW1122
JW3978
JW0710 |
