| RRC ID |
4973
|
| 著者 |
Messing SA, Gabelli SB, Liu Q, Celesnik H, Belasco JG, Piñeiro SA, Amzel LM.
|
| タイトル |
Structure and biological function of the RNA pyrophosphohydrolase BdRppH from Bdellovibrio bacteriovorus.
|
| ジャーナル |
Structure
|
| Abstract |
Until recently, the mechanism of mRNA decay in bacteria was thought to be different from that of eukaryotes. This paradigm changed with the discovery that RppH (ORF176/NudH/YgdP), an Escherichia coli enzyme that belongs to the Nudix superfamily, is an RNA pyrophosphohydrolase that initiates mRNA decay by cleaving pyrophosphate from the 5'-triphosphate. Here we report the 1.9 Angstroms resolution structure of the Nudix hydrolase BdRppH from Bdellovibrio bacteriovorus, a bacterium that feeds on other Gram-negative bacteria. Based on the structure of the enzyme alone and in complex with GTP-Mg2+, we propose a mode of RNA binding similar to that of the nuclear decapping enzyme from Xenopus laevis, X29. In additional experiments, we show that BdRppH can indeed function in vitro and in vivo as an RNA pyrophosphohydrolase. These findings set the basis for the identification of possible decapping enzymes in other bacteria.
|
| 巻・号 |
17(3)
|
| ページ |
472-81
|
| 公開日 |
2009-3-11
|
| DOI |
10.1016/j.str.2008.12.022
|
| PII |
S0969-2126(09)00075-6
|
| PMID |
19278661
|
| PMC |
PMC2993163
|
| MeSH |
Bacterial Proteins / chemistry*
Bacterial Proteins / metabolism
Bdellovibrio / enzymology*
Catalysis
Crystallography, X-Ray
Dimerization
Escherichia coli / genetics
Escherichia coli / metabolism
Models, Molecular
Protein Conformation
Pyrophosphatases / chemistry*
Pyrophosphatases / metabolism
RNA / metabolism*
RNA Stability
RNA, Bacterial / metabolism*
|
| IF |
4.862
|
| 引用数 |
28
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 原核生物(大腸菌) |
CV-25(DH5alpha)
JW2798 |
