RRC ID 4974
著者 Meyer R.
タイトル The r1162 mob proteins can promote conjugative transfer from cryptic origins in the bacterial chromosome.
ジャーナル J Bacteriol
Abstract The mobilization proteins of the broad-host-range plasmid R1162 can initiate conjugative transfer of a plasmid from a 19-bp locus that is partially degenerate in sequence. Such loci are likely to appear by chance in the bacterial chromosome and could act as cryptic sites for transfer of chromosomal DNA when R1162 is present. The R1162-dependent transfer of chromosomal DNA, initiated from one such potential site in Pectobacterium atrosepticum, is shown here. A second active site was identified in Escherichia coli, where it is also shown that large amounts of DNA are transferred. This transfer probably reflects the combined activity of the multiple cryptic origins in the chromosome. Transfer of chromosomal DNA due to the presence of a plasmid in the cytoplasm describes a previously unrecognized potential for the exchange of bacterial DNA.
巻・号 191(5)
ページ 1574-80
公開日 2009-3-1
DOI 10.1128/JB.01471-08
PII JB.01471-08
PMID 19074386
PMC PMC2648216
MeSH Bacterial Proteins / genetics Bacterial Proteins / metabolism* Base Sequence Chromosomes, Bacterial / genetics* Conjugation, Genetic* DNA, Bacterial / genetics DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Escherichia coli / genetics Molecular Sequence Data Pectobacterium / genetics Plasmids / genetics R Factors* Replication Origin / genetics*
IF 3.006
引用数 12
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(大腸菌) ME7986(MG1655) JW2128 JW2161 ME9062(BW25113)