RRC ID |
50331
|
著者 |
Ema M, Sogawa K, Watanabe N, Chujoh Y, Matsushita N, Gotoh O, Funae Y, Fujii-Kuriyama Y.
|
タイトル |
cDNA cloning and structure of mouse putative Ah receptor.
|
ジャーナル |
Biochem Biophys Res Commun
|
Abstract |
Mouse cDNA clones for a putative Ah receptor have been isolated from a cDNA library of mRNA from Hepa-1 cells by an oligonucleotide probe produced by PCR with a pair of primers which was synthesized according to the reported N-terminal sequence of 26 amino acids. The cDNA clones encode a polypeptide of 805 amino acids with a helix-loop-helix motif and with some similarity to a certain region designated PAS of Drosophila Per and Sim, and human Arnt protein. Cotransfection of an expression vector of the Ah receptor with a reporter plasmid pMC6.3k consisting of CYP1A1 promoter and CAT structural gene into CV-1 cells enhanced the CAT expression in response to added 3-methylcholanthrene.
|
巻・号 |
184(1)
|
ページ |
246-53
|
公開日 |
1992-4-15
|
DOI |
10.1016/0006-291x(92)91185-s
|
PII |
0006-291X(92)91185-S
|
PMID |
1314586
|
MeSH |
Amino Acid Sequence
Animals
Base Sequence
Cell Line
Chloramphenicol O-Acetyltransferase / genetics
Chloramphenicol O-Acetyltransferase / metabolism
Cloning, Molecular
Gene Library
Humans
Methylcholanthrene / pharmacology
Mice
Molecular Sequence Data
Oligonucleotide Probes
Polychlorinated Dibenzodioxins / metabolism
Polymerase Chain Reaction
Receptors, Aryl Hydrocarbon
Receptors, Drug / genetics*
Recombinant Fusion Proteins / metabolism
Restriction Mapping
Sequence Homology, Nucleic Acid
Transfection
|
IF |
2.985
|
引用数 |
370
|
リソース情報 |
遺伝子材料 |
Mouse Ah receptor (RDB01364) |