| RRC ID |
50621
|
| 著者 |
Sugino H, Usui T, Shimada T, Nakano M, Ogasawara H, Ishihama A, Hirata A.
|
| タイトル |
A structural sketch of RcdA, a transcription factor controlling the master regulator of biofilm formation.
|
| ジャーナル |
FEBS Lett
|
| Abstract |
RcdA is a regulator of curlin subunit gene D, the master regulator of biofilm formation in Escherichia coli. Here, we determined the X-ray structure of RcdA at 2.55 Å resolution. RcdA consists of an N-terminal DNA-binding domain (DBD) containing a helix-turn-helix (HTH) motif and a C-terminal dimerization domain, and forms a homodimer in crystals. A computational docking model of the RcdA-DNA complex allowed prediction of the candidate residues responsible for DNA binding. Our structure-guided mutagenesis, in combination with gel shift assay, atomic force microscopic observation, and reporter assay, indicate that R32 in α2 of the HTH motif plays an essential role in the recognition and binding of target DNA while T46 in α3 influences the mode of oligomerization. These results provide insights into the DNA-binding mode of RcdA.
|
| 巻・号 |
591(13)
|
| ページ |
2019-2031
|
| 公開日 |
2017-7-1
|
| DOI |
10.1002/1873-3468.12713
|
| PMID |
28608551
|
| MeSH |
Biofilms / growth & development*
DNA, Bacterial / chemistry
DNA, Bacterial / metabolism
DNA-Binding Proteins / chemistry*
DNA-Binding Proteins / metabolism*
Escherichia coli / metabolism
Escherichia coli / physiology
Escherichia coli Proteins / chemistry*
Escherichia coli Proteins / metabolism*
Models, Molecular
Nucleic Acid Conformation
Protein Conformation, alpha-Helical
Transcription Factors / chemistry*
Transcription Factors / metabolism*
|
| IF |
3.057
|
| 引用数 |
3
|
| リソース情報 |
| 原核生物(大腸菌) |
ME9780(Escherichia coli K12 W3110 type-A) |
