論文 - 詳細
RRC ID | 51244 |
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著者 | Tamada A, Igarashi M. |
タイトル | Revealing chiral cell motility by 3D Riesz transform-differential interference contrast microscopy and computational kinematic analysis. |
ジャーナル | Nat Commun |
Abstract |
Left-right asymmetry is a fundamental feature of body plans, but its formation mechanisms and roles in functional lateralization remain unclear. Accumulating evidence suggests that left-right asymmetry originates in the cellular chirality. However, cell chirality has not yet been quantitatively investigated, mainly due to the absence of appropriate methods. Here we combine 3D Riesz transform-differential interference contrast (RT-DIC) microscopy and computational kinematic analysis to characterize chiral cellular morphology and motility. We reveal that filopodia of neuronal growth cones exhibit 3D left-helical motion with retraction and right-screw rotation. We next apply the methods to amoeba Dictyostelium discoideum and discover right-handed clockwise cell migration on a 2D substrate and right-screw rotation of subcellular protrusions along the radial axis in a 3D substrate. Thus, RT-DIC microscopy and the computational kinematic analysis are useful and versatile tools to reveal the mechanisms of left-right asymmetry formation and the emergence of lateralized functions. |
巻・号 | 8(1) |
ページ | 2194 |
公開日 | 2017-12-19 |
DOI | 10.1038/s41467-017-02193-w |
PII | 10.1038/s41467-017-02193-w |
PMID | 29259161 |
PMC | PMC5736583 |
MeSH | Animals Biomechanical Phenomena / physiology Cell Culture Techniques / methods Cell Movement / physiology* Cells, Cultured Computational Biology / methods Dictyostelium / physiology Growth Cones / physiology* Hippocampus / cytology Imaging, Three-Dimensional / methods* Isomerism Mice Mice, Inbred ICR Microscopy, Interference / methods* Pseudopodia / chemistry* Pseudopodia / physiology Rotation |
IF | 12.121 |
引用数 | 11 |
リソース情報 | |
細胞性粘菌 | S00001 |