論文 - 詳細
| RRC ID | 52853 |
|---|---|
| 著者 | Shirai A, Kawaguchi T, Shimojo H, Muramatsu D, Ishida-Yonetani M, Nishimura Y, Kimura H, Nakayama JI, Shinkai Y. |
| タイトル | Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. |
| ジャーナル | Elife |
| Abstract |
SUV39H is the major histone H3 lysine 9 (H3K9)-specific methyltransferase that targets pericentric regions and is crucial for assembling silent heterochromatin. SUV39H recognizes trimethylated H3K9 (H3K9me3) via its chromodomain (CD), and enriched H3K9me3 allows SUV39H to target specific chromosomal regions. However, the detailed targeting mechanisms, especially for naïve chromatin without preexisting H3K9me3, are poorly understood. Here we show that Suv39h1's CD (Suv39h1-CD) binds nucleic acids, and this binding is important for its function in heterochromatin assembly. Suv39h1-CD had higher binding affinity for RNA than DNA, and its ability to bind nucleic acids was independent of its H3K9me3 recognition. Suv39h1 bound major satellite RNAs in vivo, and knockdown of major satellite RNAs lowered Suv39h1 retention on pericentromere. Suv39h1 mutational studies indicated that both the nucleic acid-binding and H3K9me-binding activities of Suv39h1-CD were crucial for its pericentric heterochromatin assembly. These results suggest that chromatin-bound RNAs contribute to creating SUV39H's target specificity. |
| 巻・号 | 6 |
| 公開日 | 2017-8-1 |
| DOI | 10.7554/eLife.25317 |
| PII | e25317 |
| PMID | 28760201 |
| PMC | PMC5538823 |
| MeSH | Cell Line Heterochromatin / metabolism* Histones / metabolism* Humans Methyltransferases / metabolism* Nucleic Acids / metabolism* Protein Binding Repressor Proteins / metabolism* |
| IF | 7.08 |
| 引用数 | 31 |
| リソース情報 | |
| 遺伝子材料 | pGEX6P-3-SUV39H1(39-105) (RDB15880) pGEX6P-3-SUV39H1(39-105)-4A (RDB15881) pMCs-FLAG-Suv39h1-IRES-GFP (RDB15883) pMCs-FLAG-Suv39h1-4A-IRES-GFP (RDB15884) pMCs-FLAG-Suv39h1-Y67A-IRES-GFP (RDB15885) pMCs-FLAG-Suv39h1-Y67A-4A-IRES-GFP (RDB15886) pMCs-FLAG-Suv39h1-delta N-IRES-GFP (RDB15887) pMCs-FLAG-Suv39h1-R24 K24A-IRES-GFP (RDB15888) pCAG-FLAG-IRESpuro(NRI)-mmSuv39H1 (RDB15889) pCAG-FLAG-IRESpuro (NRI)-mmSuv39H1-H324L (RDB15890) pCAG-FLAG-IRESpuro (NRI)-mmSuv39H1-4A (RDB15891) pCAG-FLAG-IRESpuro (NRI)-mmSuv39H1-Y67A (RDB15892) pCAG-FLAG-IRESpuro (NRI)-mmSuv39H1-Y67A-4A (RDB15893) pCAG-FLAG-IRESpuro (NRI)-mmSuv39H1-mut1 (RDB15894) pCAG-FLAG-IRESpuro (NRI)-mmSuv39H1-mut3 (RDB15895) pMCs-GFP-Suv39h1 (RDB15896) pMCs-GFP-Suv39h1-4A (RDB15897) pMCs-GFP-Suv39h1-Y67A (RDB15898) pMCs-GFP-Suv39h1-Y67A-4A (RDB15899) pMCs-GFP-Suv39h1-mut1 (RDB15900) pMCs-GFP-Suv39h1-mut3 (RDB15901) pGEX6P-3-SUV39H1(39-105)-Y67A (RDB15882). |