| RRC ID |
52865
|
| 著者 |
Shiomi D, Toyoda A, Aizu T, Ejima F, Fujiyama A, Shini T, Kohara Y, Niki H.
|
| タイトル |
Mutations in cell elongation genes mreB, mrdA and mrdB suppress the shape defect of RodZ-deficient cells.
|
| ジャーナル |
Mol Microbiol
|
| Abstract |
RodZ interacts with MreB and both factors are required to maintain the rod shape of Escherichia coli. The assembly of MreB into filaments regulates the subcellular arrangement of a group of enzymes that synthesizes the peptidoglycan (PG) layer. However, it is still unknown how polymerization of MreB determines the rod shape of bacterial cells. Regulatory factor(s) are likely to be involved in controlling the function and dynamics of MreB. We isolated suppressor mutations to partially recover the rod shape in rodZ deletion mutants and found that some of the suppressor mutations occurred in mreB. All of the mreB mutations were in or in the vicinity of domain IA of MreB. Those mreB mutations changed the property of MreB filaments in vivo. In addition, suppressor mutations were found in the periplasmic regions in PBP2 and RodA, encoded by mrdA and mrdB genes. Similar to MreB and RodZ, PBP2 and RodA are pivotal to the cell wall elongation process. Thus, we found that mutations in domain IA of MreB and in the periplasmic domain of PBP2 and RodA can restore growth and rod shape to ΔrodZ cells, possibly by changing the requirements of MreB in the process.
|
| 巻・号 |
87(5)
|
| ページ |
1029-44
|
| 公開日 |
2013-3-1
|
| DOI |
10.1111/mmi.12148
|
| PMID |
23301723
|
| PMC |
PMC3599482
|
| MeSH |
Cytoskeletal Proteins / deficiency*
Cytoskeletal Proteins / genetics
Down-Regulation*
Escherichia coli / cytology*
Escherichia coli / genetics
Escherichia coli / metabolism*
Escherichia coli Proteins / genetics*
Escherichia coli Proteins / metabolism
Gene Deletion
Gene Expression Regulation, Bacterial
Membrane Proteins / genetics*
Membrane Proteins / metabolism
Penicillin-Binding Proteins / genetics*
Penicillin-Binding Proteins / metabolism
Suppression, Genetic*
|
| IF |
3.418
|
| 引用数 |
36
|
|
WOS 分野
|
MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 原核生物(大腸菌) |
ME9812
ME9823
ME9824
ME9825
ME9826
ME9827
ME9829
ME9830
ME9831
ME9836
ME9837
ME9838
ME9841
ME9842
ME9844
ME9845
ME9846
ME9847
ME9848
ME9849
ME9850
ME9851
ME9855
ME9856
ME9857
ME9858
ME9863
ME9864
ME9865
ME9866
ME9867
ME9868
ME9869
ME9870
ME9871
ME9872
ME9877
ME9878 |
