RRC ID 55725
著者 Fujii T, Banno Y.
タイトル Identification of a novel function of the silkworm integument in nitrogen metabolism: Uric acid is synthesized within the epidermal cells in B. mori.
ジャーナル Insect Biochem Mol Biol
Abstract During nitrogen metabolism, animals convert toxic ammonia to less toxic forms. Uric acid (UA) is an end product of this process in terrestrial insects. In lepidopteran larvae, a large amount of UA is stored in the integument via a phenomenon known as storage excretion. Physiologically, integumental UA plays crucial roles as a barrier against sunlight and as a white pigment for larval pigmentation patterns. Conventionally, UA is thought to be synthesized in the fat body, the insect equivalent of the liver of vertebrates, and to be transported to the epidermis via the hemolymph. Here, we reconsidered the conventional theory by a mosaic analysis targeting genes governing UA synthesis, using CRISPR/Cas9 mutagenesis and a traditional genetic method in Bombyx mori. Notably, we observed mosaic larvae in which the integument comprised both UA-containing white and UA-lacking translucent areas, indicating that UA synthesis in the epidermis is indispensable to the accumulation of a large amount of highly insoluble UA in the epidermis. Our results thus provide a genetic basis for storage excretion wherein lepidopteran insects use nitrogenous waste to adapt to their environment.
巻・号 105
ページ 43-50
公開日 2019-2-1
DOI 10.1016/j.ibmb.2018.12.014
PII S0965-1748(18)30333-3
PMID 30610924
MeSH Animals Bombyx / genetics Bombyx / metabolism* Carrier Proteins / metabolism Coenzymes / metabolism Female Male Membrane Proteins / metabolism Metalloproteins / metabolism Molybdenum Cofactors Nitrogen / metabolism* Pteridines / metabolism Skin / metabolism Uric Acid / metabolism* Xanthine Dehydrogenase / metabolism
IF 3.618
引用数 3
リソース情報
カイコ p50 p55 o35 o36