| RRC ID |
56979
|
| 著者 |
Patil AA, Tatsuke T, Mon H, Lee JM, Morokuma D, Hino M, Kusakabe T.
|
| タイトル |
Characterization of Armitage and Yb containing granules and their relationship to nuage in ovary-derived cultured silkworm cell.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
PIWI-interacting RNAs (piRNAs) are a class of endogenous small non-coding RNAs, which are mostly 24-32 nucleotides in length and interact specifically with PIWI subfamily of argonaute proteins. Despite the significant research progress in germ line piRNA pathway, its role in somatic cell is not well known. In Drosophila ovarian somatic cell, maturation of primary piRNA and its loading onto Piwi occurs at perinuclear Yb body. The Armitage (Armi) and Yb proteins are the major components of Yb body and specially expressed in ovarian somatic cell. Based on the reports, here we studied the BmArmi and BmYb in Bombyx mori ovary-derived BmN4 cells expressing BmVasa. In this study, we show that BmArmi and BmYb co-localized with BmVasa at nuage. The helicase domains of BmArmi and BmYb are important for nuage localization. Moreover, RNAi of piRNA components reveal that BmArmi depend on BmAgo3 for nuage localization, and BmArmi and BmYb form cytoplasmic granules independently in the absence of BmVasa. Our results provide evidence that the BmArmi and BmYb coexist with BmVasa and play an important role in perinuclear nuage granules formation in ovary-derived BmN4 cell.
|
| 巻・号 |
490(2)
|
| ページ |
134-140
|
| 公開日 |
2017-8-19
|
| DOI |
10.1016/j.bbrc.2017.06.008
|
| PII |
S0006-291X(17)31117-8
|
| PMID |
28595904
|
| MeSH |
Animals
Bombyx / cytology*
Cells, Cultured
Cytoplasmic Granules / metabolism*
Female
Insect Proteins / metabolism*
Ovary / cytology*
RNA, Small Interfering / metabolism*
|
| IF |
2.705
|
| 引用数 |
3
|
| リソース情報 |
| カイコ |
p50
BmN4 cell |
