| RRC ID |
57020
|
| 著者 |
Haque MR, Hirowatari A, Koyanagi A, Ichinose T, Abiru M, Mohri S, Furuya S, Yamamoto K.
|
| タイトル |
Molecular characterization and expression analysis of a phosphoserine aminotransferase involving l-serine synthesis from silkworm, Bombyx mori.
|
| ジャーナル |
Arch Insect Biochem Physiol
|
| Abstract |
In this study, we identified and characterized a phosphoserine aminotransferase (bmPSAT) from Bombyx mori (B. mori) that is responsible for l-serine biosynthesis. A complementary DNA that encodes bmPSAT was cloned by reverse transcriptase polymerase reaction and sequenced. The presumed amino acid sequence revealed 47-87% identity with known PSATs from insects, humans, plants, and bacteria. Through phylogenetic analysis, we found that bmPSAT is evolutionary related to insect PSATs. Recombinant bmPSAT was produced in Escherichia coli by using a cold-shock promotor and purified to homogeneity. This enzyme utilizes phosphohydroxypyruvate and glutamate for transamination. bmPSAT messenger RNA (mRNA) was expressed at higher levels in several tissues of standard strain silkworm including the silk gland, whereas a sericin-deficient silkworm strain exhibited a diminished expression of bmPSAT mRNA in the silk gland. These findings indicate that bmPSAT may play an important role in synthesizing and supplying l-serine in the larva of B. mori.
|
| 巻・号 |
101(2)
|
| ページ |
e21553
|
| 公開日 |
2019-6-1
|
| DOI |
10.1002/arch.21553
|
| PMID |
31004387
|
| MeSH |
Animals
Bombyx / enzymology*
Bombyx / genetics
Bombyx / metabolism
Cloning, Molecular
DNA, Complementary / genetics
Escherichia coli / genetics
Gene Expression Regulation, Developmental
Insect Proteins / biosynthesis
Insect Proteins / metabolism
Larva / metabolism
Phylogeny
Recombinant Proteins / metabolism
Serine / biosynthesis*
Transaminases / chemistry*
Transaminases / genetics
Transaminases / metabolism
|
| IF |
1.198
|
| 引用数 |
0
|
| リソース情報 |
| カイコ |
p50T
b94 |
