RRC ID 58235
著者 Iwabuchi A, Katte N, Suwa M, Goto J, Inui H.
タイトル Factors regulating the differential uptake of persistent organic pollutants in cucurbits and non-cucurbits.
ジャーナル J Plant Physiol
Abstract Contamination with persistent organic pollutants (POPs) has become a worldwide concern owing to their the toxicity to humans and wildlife. Pumpkin, cucumber, and squash (Cucurbitaceae) accumulate POPs in their shoots in concentrations higher than those in non-cucurbits; to elucidate the underlying molecular mechanisms of this accumulation, POP transporters were analyzed in the xylem sap of cucurbits and non-cucurbits. The 17-kDa xylem sap proteins detected in all cucurbits but not in non-cucurbits readily bound polychlorinated biphenyl (PCB) in all tested cucurbits, except in cucumber and loofah, and to dieldrin in all tested cucurbits. Ten genes encoding major latex-like proteins (MLPs) responsible for the accumulation of PCBs in zucchini plants were cloned from cucurbits. Phylogenetic analysis using MLP sequences identified two separate clades, one containing Cucurbitaceae MLPs and the other containing those of non-cucurbit members. Recombinant MLPs bound PCB and dieldrin. Western blotting with anti-MLP antibodies identified translocatable and non-translocatable MLPs between root and stem xylem vessels. Translocation of MLPs from the root to stem xylem vessels and POP-binding ability of MLPs are important for selective accumulation of MLPs in cucurbits. This study provides basic knowledge about phytoremediation through overexpression of MLP genes and for breeding cucurbits that accumulate less contaminants.
巻・号 245
ページ 153094
公開日 2020-2-1
DOI 10.1016/j.jplph.2019.153094
PII S0176-1617(19)30223-8
PMID 31862647
MeSH Biodegradation, Environmental Biological Transport / genetics Biological Transport / physiology Cucurbitaceae / metabolism* Environmental Pollutants / chemistry Environmental Pollutants / metabolism Gene Expression / genetics Phylogeny Plant Proteins / genetics Plant Proteins / isolation & purification Plant Proteins / metabolism* Plant Roots / metabolism Plants, Genetically Modified Polychlorinated Biphenyls / metabolism Protein Binding / genetics Soil Pollutants / chemistry Soil Pollutants / metabolism* Xylem / metabolism
IF 2.825
引用数 1
リソース情報
トマト TOMJPF00001 (Micro-Tom)