RRC ID 58366
著者 Shichino Y, Otsubo Y, Yamamoto M, Yamashita A.
タイトル Meiotic gene silencing complex MTREC/NURS recruits the nuclear exosome to YTH-RNA-binding protein Mmi1.
ジャーナル PLoS Genet
Abstract Accurate target recognition in transcript degradation is crucial for regulation of gene expression. In the fission yeast Schizosaccharomyces pombe, a number of meiotic transcripts are recognized by a YTH-family RNA-binding protein, Mmi1, and selectively degraded by the nuclear exosome during mitotic growth. Mmi1 forms nuclear foci in mitotically growing cells, and the nuclear exosome colocalizes to such foci. However, it remains elusive how Mmi1 and the nuclear exosome are connected. Here, we show that a complex called MTREC (Mtl1-Red1 core) or NURS (nuclear RNA silencing) that consists of a zinc-finger protein, Red1, and an RNA helicase, Mtl1, is required for the recruitment of the nuclear exosome to Mmi1 foci. Physical interaction between Mmi1 and the nuclear exosome depends on Red1. Furthermore, a chimeric protein involving Mmi1 and Rrp6, which is a nuclear-specific component of the exosome, suppresses the ectopic expression phenotype of meiotic transcripts in red1Δ cells and mtl1 mutant cells. These data indicate that the primary function of MTREC/NURS in meiotic transcript elimination is to link Mmi1 to the nuclear exosome physically.
巻・号 16(2)
ページ e1008598
公開日 2020-2-1
DOI 10.1371/journal.pgen.1008598
PII PGENETICS-D-19-01079
PMID 32012158
PMC PMC7018101
MeSH Carrier Proteins / metabolism* DEAD-box RNA Helicases / metabolism Exosomes / metabolism* Gene Expression Regulation, Fungal* Meiosis / genetics* RNA Interference* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Ribonucleases / genetics Ribonucleases / metabolism Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* mRNA Cleavage and Polyadenylation Factors / genetics mRNA Cleavage and Polyadenylation Factors / metabolism*
IF 5.175
引用数 0
リソース情報
酵母 FY24020(JY450)