RRC ID |
59819
|
著者 |
Takada H, Fukushima-Tanaka S, Morita M, Kasahara Y, Watanabe S, Chibazakura T, Hara H, Matsumoto K, Yoshikawa H.
|
タイトル |
An essential enzyme for phospholipid synthesis associates with the Bacillus subtilis divisome.
|
ジャーナル |
Mol Microbiol
|
Abstract |
The mechanism by which the membrane synthetic machinery might be co-organized with the cell-division architecture during the bacterial cell cycle remains to be investigated. We characterized a key enzyme of phospholipid and fatty acid synthesis in Bacillus subtilis, the acyl-acyl carrier protein phosphate acyltransferase (PlsX), and identified it as a component of the cell-division machinery. Comprehensive interaction analysis revealed that PlsX interacts with FtsA, the FtsZ-anchoring protein. PlsX mainly localized at the potential division site independent of FtsA and FtsZ and then colocalized with FtsA. By multidirectional approaches, we revealed that the Z-ring stabilizes the association of PlsX at the septum and pole. The localization of PlsX is also affected by the progression of DNA replication. PlsX is needed for cell division and its inactivation leads to aberrant Z-ring formation. We propose that PlsX localization is prior to Z-ring formation in the hierarchy of septum formation events and that PlsX is important for co-ordinating membrane synthesis with cell division in order to properly complete septum formation.
|
巻・号 |
91(2)
|
ページ |
242-55
|
公開日 |
2014-1-1
|
DOI |
10.1111/mmi.12457
|
PMID |
24224907
|
MeSH |
Bacillus subtilis / cytology
Bacillus subtilis / enzymology*
Bacillus subtilis / genetics
Bacterial Proteins / chemistry*
Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
Cell Division
Cell Polarity
Cytoskeletal Proteins / metabolism*
DNA Replication
DNA, Bacterial / physiology
Enzyme Stability
|
IF |
3.649
|
引用数 |
22
|
リソース情報 |
原核生物(枯草菌) |
MBS699
MBS700
MBS701
MBS702
MBS703
MBS704
MBS705
MBS706
MBS707
MBS708
MBS709
MBS710
MBS711
MBS712
MBS713
MBS714
MBS715
MBS716
MBS717
MBS718
MBS719
MBS720
MBS721
MBS722
MBS723
MBS724
MBS725
MBS726
MBS727
MBS728
MBS890
MBS891
MBS892
MBS893
MBS894
MBS895
MBS896
MBS897
MBS898
MBS899
MBS900
MBS901 |