RRC ID 6189
著者 Cavaco LM, Hasman H, Xia S, Aarestrup FM.
タイトル qnrD, a novel gene conferring transferable quinolone resistance in Salmonella enterica serovar Kentucky and Bovismorbificans strains of human origin.
ジャーナル Antimicrob Agents Chemother
Abstract In a previous study, four Salmonella isolates from humans in the Henan province of China showed reduced susceptibility to ciprofloxacin (MIC, 0.125 to 0.25 microg/ml) but were susceptible to nalidixic acid (MIC, 4 to 8 microg/ml). All isolates were negative for known qnr genes (A, B, and S), aac(6')Ib-cr, and mutations in gyrA and parC. Plasmid DNA was extracted from all four isolates and transformed into Escherichia coli TG1 and DH10B cells by electroporation, and transformants were selected on 0.06 microg/ml ciprofloxacin containing brain heart infusion agar plates. Resistance to ciprofloxacin could be transferred by electroporation, and a similar 4,270-bp plasmid was found in all transformants. By sequence analysis, the plasmid was found to carry an open reading frame that had similarities to other qnr genes and that encoded a 214-amino-acid pentapeptide repeat protein. This gene, designated qnrD, showed 48% similarity to qnrA1, 61% similarity to qnrB1, and 41% similarity to qnrS1. Further subcloning of the qnrD coding region into the constitutively expressed tetA gene of vector pBR322 showed that the gene conferred an increase in the MIC of ciprofloxacin by a factor of 32 (from an MIC of 0.002 to an MIC of 0.06 microg/ml). For comparison, qnrA1 and qnrS1 were also subcloned into pBR322 and transformed into DH10B cells, conferring MICs of 0.125 and 0.5 microg/ml, respectively. A phylogenetic analysis of all known qnr sequences was performed and showed that qnrD was more closely related to the qnrB variants but formed an independent cluster. To our knowledge, this is the first description of this qnrD gene.
巻・号 53(2)
ページ 603-8
公開日 2009-2-1
DOI 10.1128/AAC.00997-08
PII AAC.00997-08
PMID 19029321
PMC PMC2630628
MeSH Amino Acid Sequence Animals Anti-Bacterial Agents / pharmacology* Cattle Cloning, Molecular DNA, Bacterial / genetics Drug Resistance, Bacterial / genetics* Escherichia coli / genetics Humans Microbial Sensitivity Tests Molecular Sequence Data Phylogeny Plasmids / genetics Quinolones / pharmacology* Salmonella enterica / drug effects* Salmonella enterica / genetics*
IF 4.904
引用数 322
WOS 分野 PHARMACOLOGY & PHARMACY MICROBIOLOGY
リソース情報
遺伝子材料 pBC-H2.6 (RDB05463)