RRC ID 6718
著者 Morita T, Yamaguchi H, Amagai A, Maeda Y.
タイトル Involvement of the TRAP-1 homologue, Dd-TRAP1, in spore differentiation during Dictyostelium development.
ジャーナル Exp Cell Res
Abstract Tumor necrosis factor receptor-associated protein 1 (TRAP1) is a member of the molecular chaperone HSP90 (90-kDa heat shock protein) family. We have previously demonstrated that Dictyostelium discoideum TRAP1 (Dd-TRAP1) synthesized at the vegetative growth phase is retained during the whole course of D. discoideum development, and that at the multicellular slug stage, it is located in prespore-specific vacuoles (PSVs) of prespore cells as well as in the cell membrane and mitochondria. Thereupon, we examined the function of Dd-TRAP1 in prepore and spore differentiation, using Dd-TRAP1-knockdown cells (TRAP1-RNAi cells) produced by the RNA interference method. As was expected, Dd-TRAP1 contained in the PSV was found to be exocytosed during sporulation to constitute the outer-most layer of the spore cell wall. In the TRAP1-RNAi cells, PSV formation and therefore prespore differentiation were significantly impaired, particularly under a heat stress condition. Although the TRAP1-RNAi cells formed apparently normal-shaped spores with a cellulosic wall, the spores were less resistant to heat and detergent treatments, as compared with those of parental MB35 cells derived from Ax-2 cells. These findings strongly suggest that Dd-TRAP1 may be closely involved in late development including spore differentiation, as well as in early development as realized by its induction of prestarvation response.
巻・号 303(2)
ページ 425-31
公開日 2005-2-15
DOI 10.1016/j.yexcr.2004.10.010
PII S0014-4827(04)00615-9
PMID 15652354
MeSH Animals Cell Wall / metabolism Cell Wall / ultrastructure Dictyostelium / genetics Dictyostelium / growth & development* Dictyostelium / metabolism* Dictyostelium / ultrastructure HSP90 Heat-Shock Proteins / genetics HSP90 Heat-Shock Proteins / metabolism* Heat-Shock Response Microscopy, Immunoelectron Protozoan Proteins / genetics Protozoan Proteins / metabolism* RNA Interference Spores / growth & development Spores / metabolism Spores / ultrastructure
IF 3.383
引用数 16
WOS 分野 ONCOLOGY CELL BIOLOGY
リソース情報
細胞性粘菌 G01070 S90287 S90288