RRC ID |
6729
|
著者 |
Chubb JR, Bloomfield G, Xu Q, Kaller M, Ivens A, Skelton J, Turner BM, Nellen W, Shaulsky G, Kay RR, Bickmore WA, Singer RH.
|
タイトル |
Developmental timing in Dictyostelium is regulated by the Set1 histone methyltransferase.
|
ジャーナル |
Dev Biol
|
Abstract |
Histone-modifying enzymes have enormous potential as regulators of the large-scale changes in gene expression occurring during differentiation. It is unclear how different combinations of histone modification coordinate regimes of transcription during development. We show that different methylation states of lysine 4 of histone H3 (H3K4) mark distinct developmental phases of the simple eukaryote, Dictyostelium. We demonstrate that the enzyme responsible for all mono, di and tri-methylation of H3K4 is the Dictyostelium homolog of the Set1 histone methyltransferase. In the absence of Set1, cells display unusually rapid development, characterized by precocious aggregation of amoebae into multicellular aggregates. Early differentiation markers are abundantly expressed in growing set1 cells, indicating the differentiation program is ectopically activated during growth. This phenotype is caused specifically by the loss of Set1 catalytic activity. Set1 mutants induce premature differentiation in wild-type cells, indicating Set1 regulates production of an extra-cellular factor required for the correct perception of growth conditions. Microarray analysis of the set1 mutants reveals genomic clustering of mis-expressed genes, suggesting a requirement for Set1 in the regulation of chromatin-mediated events at gene clusters.
|
巻・号 |
292(2)
|
ページ |
519-32
|
公開日 |
2006-4-15
|
DOI |
10.1016/j.ydbio.2005.12.054
|
PII |
S0012-1606(06)00013-3
|
PMID |
16469305
|
MeSH |
Amino Acid Sequence
Animals
Biomarkers / metabolism
Blotting, Western
Cell Differentiation
Chromatin Immunoprecipitation
Cluster Analysis
Dictyostelium / growth & development*
Gene Expression Profiling
Gene Expression Regulation, Developmental*
Genes, Protozoan
Genome, Protozoan
Genomics
Histone Methyltransferases
Histone-Lysine N-Methyltransferase / chemistry
Histone-Lysine N-Methyltransferase / genetics
Histone-Lysine N-Methyltransferase / metabolism*
Histones / chemistry
Histones / metabolism*
Luminescent Measurements
Lysine / metabolism
Methylation
Molecular Sequence Data
Mutation
Oligonucleotide Array Sequence Analysis
Phylogeny
Protein Methyltransferases
Protein Structure, Tertiary
Protozoan Proteins / chemistry
Protozoan Proteins / genetics
Protozoan Proteins / metabolism*
Sequence Homology, Amino Acid
Time Factors
Transcription, Genetic
|
IF |
2.896
|
引用数 |
26
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
|
リソース情報 |
細胞性粘菌 |
G03274
S00451 |