RRC ID 67579
著者 Ebihara T, Xu J, Tonooka Y, Nagasato T, Kakino K, Masuda A, Minamihata K, Kamiya N, Nakatake H, Chieda Y, Mon H, Fujii T, Kusakabe T, Lee JM.
タイトル Active Human and Murine Tumor Necrosis Factor α Cytokines Produced from Silkworm Baculovirus Expression System.
ジャーナル Insects
Abstract The tumor necrosis factor α (TNFα) has been employed as a promising reagent in treating autoimmunity and cancer diseases. To meet the substantial requirement of TNFα proteins, we report in this study that mature types of recombinant human and murine TNFα proteins are successfully expressed in the baculovirus expression system using silkworm larvae as hosts. The biological activities of purified products were verified in culture murine L929 cells, showing better performance over a commercial Escherichia coli-derived murine TNFα. By comparing the activity of purified TNFα with or without the tag removal, it is also concluded that the overall activity of purified TNFα cytokines could be further improved by the complete removal of C-terminal fusion tags. Collectively, our current attempt demonstrates an alternative platform for supplying high-quality TNFα products with excellent activities for further pharmaceutical and clinical trials.
巻・号 12(6)
公開日 2021-6-2
DOI 10.3390/insects12060517
PII insects12060517
PMID 34199525
PMC PMC8230043
IF 2.22
リソース情報
ヒト・動物細胞 L929(RCB2619)
カイコ k45