| RRC ID |
71812
|
| 著者 |
Tomihara K, Satta K, Matsuzaki S, Yoshitake K, Yamamoto K, Uchiyama H, Yajima S, Futahashi R, Katsuma S, Osanai-Futahashi M, Kiuchi T.
|
| タイトル |
Mutations in a β-group of solute carrier gene are responsible for egg and eye coloration of the brown egg 4 (b-4) mutant in the silkworm, Bombyx mori.
|
| ジャーナル |
Insect Biochem Mol Biol
|
| Abstract |
The brown egg 4 (b-4) is a recessive mutant in the silkworm (Bombyx mori), whose egg and adult compound eyes exhibit a reddish-brown color instead of normal purple and black, respectively. By double digest restriction-site associated DNA sequencing (ddRAD-seq) analysis, we narrowed down a region linked to the b-4 phenotype to approximately 1.1 Mb that contains 69 predicted gene models. RNA-seq analysis in a b-4 strain indicated that one of the candidate genes had a different transcription start site, which generates a short open reading frame. We also found that exon skipping was induced in the same gene due to an insertion of a transposable element in other two b-4 mutant strains. This gene encoded a putative amino acid transporter that belongs to the β-group of solute carrier (SLC) family and is orthologous to Drosophila eye color mutant gene, mahogany (mah). Accordingly, we named this gene Bmmah. We performed CRISPR/Cas9-mediated gene knockout targeting Bmmah. Several adult moths in generation 0 (G0) had totally or partially reddish-brown compound eyes. We also established three Bmmah knockout strains, all of which exhibit reddish-brown eggs and adult compound eyes. Furthermore, eggs from complementation crosses between the b-4 mutants and the Bmmah knockout mutants also exhibited reddish-brown color, which was similar to the b-4 mutant eggs, indicating that Bmmah is responsible for the b-4 phenotypes.
|
| 巻・号 |
137
|
| ページ |
103624
|
| 公開日 |
2021-10-1
|
| DOI |
10.1016/j.ibmb.2021.103624
|
| PII |
S0965-1748(21)00107-7
|
| PMID |
34333110
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
Bombyx / genetics*
Bombyx / growth & development
Bombyx / metabolism
Compound Eye, Arthropod / chemistry*
Insect Proteins / chemistry
Insect Proteins / genetics*
Insect Proteins / metabolism
Larva / growth & development
Larva / metabolism
Mutation
Ovum / chemistry
Phylogeny
Pigmentation / genetics
Pigments, Biological / analysis
Sequence Alignment
|
| IF |
3.827
|
| リソース情報 |
| カイコ |
e36,e37 |
