| RRC ID |
7187
|
| 著者 |
Hirai T, Fukukawa G, Kakuta H, Fukuda N, Ezura H.
|
| タイトル |
Production of recombinant miraculin using transgenic tomatoes in a closed cultivation system.
|
| ジャーナル |
J Agric Food Chem
|
| Abstract |
We constructed a cultivation system with a controlled light period, light intensity, temperature, and CO(2) concentration for mass production of the taste-modifying protein miraculin from transgenic tomatoes. The tomato plants exhibited normal growth and produced over 270 g of fresh weight (FW) fruit per plant, with the recombinant miraculin concentration reaching up to 90 microg per g FW of tomatoes. The recombinant miraculin content of transgenic tomatoes was compared to that of plants grown in a netted greenhouse. The recombinant miraculin content of transgenic tomatoes grown in a closed cultivation system was more stable than that of tomatoes grown in a netted greenhouse, suggesting that the closed cultivation system is suitable for the production of recombinant miraculin. We estimate that 45 tFW of tomatoes and 4 kg of recombinant miraculin per 1,000 m(2) of cultivation area can be harvested per year.
|
| 巻・号 |
58(10)
|
| ページ |
6096-101
|
| 公開日 |
2010-5-26
|
| DOI |
10.1021/jf100414v
|
| PMID |
20426470
|
| MeSH |
Agriculture / methods
Environment, Controlled
Fruit / chemistry
Fruit / metabolism
Glycoproteins / analysis
Glycoproteins / biosynthesis*
Glycoproteins / genetics
Lycopersicon esculentum / genetics
Lycopersicon esculentum / growth & development*
Lycopersicon esculentum / metabolism*
Plants, Genetically Modified / metabolism*
Recombinant Proteins / analysis
Recombinant Proteins / biosynthesis*
|
| IF |
4.192
|
| 引用数 |
21
|
|
WOS 分野
|
AGRICULTURE, MULTIDISCIPLINARY
FOOD SCIENCE & TECHNOLOGY
CHEMISTRY, APPLIED
|
| リソース情報 |
| トマト |
TOMJPF00002(Moneymaker) |
