| RRC ID |
894
|
| 著者 |
Mitsui K, Ochi F, Nakamura N, Doi Y, Inoue H, Kanazawa H.
|
| タイトル |
A novel membrane protein capable of binding the Na+/H+ antiporter (Nha1p) enhances the salinity-resistant cell growth of Saccharomyces cerevisiae.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
The Na+/H+ antiporter Nha1p of Saccharomyces cerevisiae plays an important role in maintaining intracellular pH and Na+ homeostasis. Nha1p has a two-domain structure composed of integral membrane and hydrophilic tail regions. Overexpression of a peptide of approximately 40 residues (C1+C2 domains) that is localized in the juxtamembrane area of its cytoplasmic tail caused cell growth retardation in highly saline conditions, possibly by decreasing Na+/H+ antiporter activity. A multicopy suppressor gene of this growth retardation was identified from a yeast genome library. The clone encodes a novel membrane protein denoted as COS3 in the genome data base. Overexpression or deletion of COS3 increases or decreases salinity-resistant cell growth, respectively. However, in nha1Delta cells, overexpression of COS3 alone did not suppress the growth retardation. Cos3p and a hydrophilic portion of Cos3p interact with the C1+C2 peptide in vitro, and Cos3p is co-precipitated with Nha1p from yeast cell extracts. Cos3p-GFP mainly resides at the vacuole, but overexpression of Nha1p caused a portion of the Cos3p-GFP proteins to shift to the cytoplasmic membrane. These observations suggest that Cos3p is a novel membrane protein that can enhance salinity-resistant cell growth by interacting with the C1+C2 domain of Nha1p and thereby possibly activating the antiporter activity of this protein.
|
| 巻・号 |
279(13)
|
| ページ |
12438-47
|
| 公開日 |
2004-3-26
|
| DOI |
10.1074/jbc.M310806200
|
| PII |
S0021-9258(19)64180-3
|
| PMID |
14718542
|
| MeSH |
Amino Acid Sequence
Cation Transport Proteins / chemistry*
Cation Transport Proteins / metabolism*
Cell Division
Cell Membrane / metabolism*
Centrifugation, Density Gradient
Cytoplasm / metabolism
DNA Primers / pharmacology
Dose-Response Relationship, Drug
Gene Library
Genes, Dominant
Genes, Fungal
Green Fluorescent Proteins
Luminescent Proteins / metabolism
Membrane Proteins / chemistry*
Membrane Proteins / metabolism*
Membrane Proteins / physiology*
Molecular Sequence Data
Open Reading Frames
Peptides / chemistry
Plasmids / metabolism
Precipitin Tests
Protein Binding
Protein Structure, Tertiary
Saccharomyces cerevisiae / metabolism*
Saccharomyces cerevisiae Proteins / chemistry*
Saccharomyces cerevisiae Proteins / physiology*
Sequence Homology, Amino Acid
Sodium Chloride / pharmacology*
Sodium-Hydrogen Exchangers / chemistry*
Sodium-Hydrogen Exchangers / metabolism*
Subcellular Fractions
Time Factors
|
| IF |
4.238
|
| 引用数 |
17
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 酵母 |
BY4849(W303-1B) |
