論文 - 詳細
| RRC ID | 1022 |
|---|---|
| 著者 | Owhashi M, Taoka Y, Ishii K, Nakazawa S, Uemura H, Kambara H. |
| タイトル | Identification of a ubiquitin family protein as a novel neutrophil chemotactic factor. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Neutrophil chemotaxis is a process that is essential for the recruitment of neutrophils to an inflamed site. In the present study, we found a remarkable increase in neutrophil chemotactic activity in the lysate of red blood cells (RBC) of mice infected with murine malaria, Plasmodium yoelii. A neutrophil chemotactic factor with an apparent molecular weight of 17 kDa (IP17) was isolated from RBC by a combination of anion-exchange chromatography on DE52 and cation-exchange chromatography on Mono S. A comprehensive GenBank database search of N-terminal amino acid sequences and MALDI-TOF mass analysis of IP17 revealed that IP17 is identical to a murine homologue of ISG15/UCRP, a member of the ubiquitin family of proteins that are inducible by interferon-beta. Recombinant mouse ISG15 showed neutrophil chemotactic activity comparable to that of natural IP17. IP17 showed specific chemotactic activity forward neutrophils and activated neutrophils to induce the release of eosinophil chemotactic factors. These results suggest that the ubiquitin family protein ISG15/UCRP has novel functions in neutrophil-mediated immune mechanisms. |
| 巻・号 | 309(3) |
| ページ | 533-9 |
| 公開日 | 2003-9-26 |
| DOI | 10.1016/j.bbrc.2003.08.038 |
| PII | S0006291X03015493 |
| PMID | 12963022 |
| MeSH | Amino Acid Sequence Animals Chemotactic Factors / chemistry Chemotactic Factors / classification Chemotactic Factors / pharmacology* Chemotaxis Cytokines / chemistry Cytokines / classification Cytokines / pharmacology* Female Humans Mice Mice, Inbred C57BL Molecular Sequence Data Neutrophils / drug effects Neutrophils / immunology* Sequence Alignment Ubiquitins / chemistry Ubiquitins / classification Ubiquitins / pharmacology* |
| IF | 2.985 |
| 引用数 | 56 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| 病原微生物 | P. yoeli? |