論文 - 詳細
| RRC ID | 10770 |
|---|---|
| 著者 | Aburai N, Yoshida M, Ohnishi M, Kimura K. |
| タイトル | Sanguinarine as a potent and specific inhibitor of protein phosphatase 2C in vitro and induces apoptosis via phosphorylation of p38 in HL60 cells. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
Sanguinarine, a plant alkaloid, was identified as a potent and specific protein phosphatase (PP) 2C inhibitor. It inhibited PP2C competitively with respect to alpha-casein (Ki=0.68 microM) and showed selectivity for PP2C as compared with PP1, PP2A, and PP2B in vitro. In vivo, sanguinarine showed cytotoxicity toward human promyelocytic leukemia cell line HL60, with an IC(50) value of 0.37 microM, and induced apoptosis through a caspase-3/7-dependent mechanism involving the phosphorylation of p38, a PP2Calpha substrate. The apoptosis activity induced by sanguinarine was partially inhibited by a p38 inhibitor, SB203580, and was involved in the phospho-p38 protein in HL60 cells. |
| 巻・号 | 74(3) |
| ページ | 548-52 |
| 公開日 | 2010-1-1 |
| DOI | 10.1271/bbb.90735 |
| PII | JST.JSTAGE/bbb/90735 |
| PMID | 20208361 |
| MeSH | Apoptosis* Benzophenanthridines / pharmacology* Caspase 3 / metabolism Caspase 7 / metabolism Enzyme Inhibitors / pharmacology* HL-60 Cells Humans Imidazoles / pharmacology Isoquinolines / pharmacology* Phosphoprotein Phosphatases / antagonists & inhibitors* Phosphorylation Protein Phosphatase 2C Pyridines / pharmacology p38 Mitogen-Activated Protein Kinases / metabolism |
| IF | 1.516 |
| 引用数 | 45 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | HL60(RCB0041) |