| RRC ID |
10899
|
| 著者 |
Joung CH, Shin JY, Koo JK, Lim JJ, Wang JS, Lee SJ, Tan HK, Kim SL, Lim SM.
|
| タイトル |
Production and characterization of long-acting recombinant human albumin-EPO fusion protein expressed in CHO cell.
|
| ジャーナル |
Protein Expr Purif
|
| Abstract |
A long-lasting recombinant human albumin-linker-erythropoietin (EPO) is a human albumin gene fused to the N-terminal of EPO with a (GGSGG)(n)-repeated linker inserted between albumin and EPO. Albumin-EPO fusion genes were co-transfected with the dhfr gene. Albumin-EPO fusion protein has three kinds of sub-types (IALE, AD2LE, AD1LE). Albumin-EPO fusion protein was quantified with human EPO ELISA. The in vitro efficacy of albumin-EPO fusion protein was estimated using F-36E cell, and in vivo efficacy of albumin-EPO fusion protein was estimated using normocythemic mice (B6D2F1). We also determined the in vivo half-life in a Sprague-Dawley rat. A PLA program analysis result demonstrated that the albumin-EPO fusion protein IALE is about 7.8-fold more potent than rHuEPO in increasing the hematocrit of normal mice.
|
| 巻・号 |
68(2)
|
| ページ |
137-45
|
| 公開日 |
2009-12-1
|
| DOI |
10.1016/j.pep.2009.07.003
|
| PII |
S1046-5928(09)00175-2
|
| PMID |
19595770
|
| MeSH |
Analysis of Variance
Animals
Blotting, Western
CHO Cells
Cell Count
Cell Line, Tumor
Chromatography, Gel
Cloning, Molecular / methods*
Cricetinae
Cricetulus
Erythropoietin / chemistry
Erythropoietin / genetics
Erythropoietin / metabolism*
Half-Life
Humans
Mice
Polymerase Chain Reaction
Rats
Recombinant Fusion Proteins / biosynthesis*
Recombinant Fusion Proteins / chemistry
Recombinant Fusion Proteins / genetics
Recombinant Proteins
Reticulocytes
Serum Albumin / biosynthesis*
Serum Albumin / chemistry
Serum Albumin / genetics
|
| IF |
1.513
|
| 引用数 |
23
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMICAL RESEARCH METHODS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
F-36E(RCB0776) |
