論文 - 詳細
RRC ID | 11216 |
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著者 | Kenzaka T, Ishidoshiro A, Tani K, Nasu M. |
タイトル | Scanning electron microscope imaging of bacteria based on DNA sequence. |
ジャーナル | Lett Appl Microbiol |
Abstract |
AIMS:To develop a scanning electron microscopic approach using in situ hybridization (SEM-ISH) for gaining both genetic and morphological information about target bacteria. METHODS AND RESULTS:Target cells were hybridized with DNA-targeted polynucleotide probes, and a tyramide signal amplification system was used to increase the sensitivity. The protocol of SEM-ISH enabled to detect low copy number target DNA sequences in individual cells. CONCLUSIONS:SEM-ISH allowed the in situ detection of bacteria carrying a specific gene. SIGNIFICANCE AND IMPACT OF THE STUDY:Combining morphological study with SEM and ISH techniques appears to be a valuable tool to understand the spatial distribution of target cells in complex microbial communities on various materials. |
巻・号 | 49(6) |
ページ | 796-9 |
公開日 | 2009-12-1 |
DOI | 10.1111/j.1472-765X.2009.02680.x |
PII | LAM2680 |
PMID | 19889108 |
MeSH | Bacteria / genetics Bacteria / isolation & purification* Bacteria / ultrastructure DNA Probes DNA, Bacterial / analysis Microscopy, Electron, Scanning / methods* Nucleic Acid Amplification Techniques Nucleic Acid Hybridization |
IF | 2.173 |
引用数 | 3 |
WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
リソース情報 | |
一般微生物 | JCM 1234 |