| RRC ID |
1188
|
| 著者 |
Aoki N, Kimura S, Oikawa K, Nochi H, Atsuta Y, Kobayashi H, Sato K, Katagiri M.
|
| タイトル |
DAP12 ITAM motif regulates differentiation and apoptosis in M1 leukemia cells.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
DAP12 is an immunoreceptor tyrosine-based activation motif (ITAM)-bearing transmembrane adapter molecule that is associated with the NK-activating receptors. DAP12 is expressed not only in NK cells, but also in myeloid cells. Previously, we reported that DAP12 was likely to be involved in monocyte differentiation to macrophage. In this study, we established the mutant DAP12-M1 transfectants (Y76F-M1) that have mutation at their ITAM motifs. We observed that Y76F-M1 cells could not differentiate to macrophages by stimulation via DAP12, whereas wild type DAP12 transfectants (FDAP-M1) could. Furthermore, we demonstrated that the apoptosis signal mediated by LPS was inhibited in Y76F-M1 cells, but was augmented in FDAP-M1 cells. In contrast to the LPS-mediated apoptosis, the combination of LPS and DAP12 stimulation showed good cell viability in FDAP-M1 cells. Collectively our studies demonstrated that DAP12 has a critical role for macrophage differentiation and LPS induced apoptosis in M1 leukemia cells.
|
| 巻・号 |
291(2)
|
| ページ |
296-304
|
| 公開日 |
2002-2-22
|
| DOI |
10.1006/bbrc.2002.6434
|
| PII |
S0006291X0296434X
|
| PMID |
11846404
|
| MeSH |
Adaptor Proteins, Signal Transducing
Amino Acid Motifs
Animals
Apoptosis*
Cell Differentiation
Cell Division
Kinetics
Leukemia, Myeloid, Acute / genetics
Leukemia, Myeloid, Acute / metabolism
Leukemia, Myeloid, Acute / pathology*
Lipopolysaccharides / antagonists & inhibitors
Macrophages / cytology
Macrophages / physiology
Membrane Proteins
Mice
Mutation
RNA, Neoplasm / biosynthesis
Receptors, Immunologic / chemistry*
Receptors, Immunologic / genetics
Receptors, Immunologic / physiology*
Signal Transduction
Transfection
Tumor Cells, Cultured
|
| IF |
2.985
|
| 引用数 |
9
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
M1(RCB0169) |
