論文 - 詳細
| RRC ID | 12124 |
|---|---|
| 著者 | Sinha DK, Neveu P, Gagey N, Aujard I, Le Saux T, Rampon C, Gauron C, Kawakami K, Leucht C, Bally-Cuif L, Volovitch M, Bensimon D, Jullien L, Vriz S. |
| タイトル | Photoactivation of the CreER T2 recombinase for conditional site-specific recombination with high spatiotemporal resolution. |
| ジャーナル | Zebrafish |
| Abstract |
We implemented a noninvasive optical method for the fast control of Cre recombinase in single cells of a live zebrafish embryo. Optical uncaging of the caged precursor of a nonendogeneous steroid by one- or two-photon illumination was used to restore Cre activity of the CreER(T2) fusion protein in specific target cells. This method labels single cells irreversibly by inducing recombination in an appropriate reporter transgenic animal and thereby can achieve high spatiotemporal resolution in the control of gene expression. This technique could be used more generally to investigate important physiological processes (e.g., in embryogenesis, organ regeneration, or carcinogenesis) with high spatiotemporal resolution (single cell and 10-min scales). |
| 巻・号 | 7(2) |
| ページ | 199-204 |
| 公開日 | 2010-6-1 |
| DOI | 10.1089/zeb.2009.0632 |
| PMID | 20441524 |
| MeSH | Animals Animals, Genetically Modified DNA Primers / genetics Gene Expression Regulation, Enzymologic / physiology* HSP70 Heat-Shock Proteins / metabolism Integrases / metabolism* Microscopy, Fluorescence Photochemical Processes Polymerase Chain Reaction Recombinant Fusion Proteins / metabolism* Recombination, Genetic / physiology* Spectrometry, Fluorescence Zebrafish* |
| IF | 1.845 |
| 引用数 | 39 |
| WOS 分野 | ZOOLOGY DEVELOPMENTAL BIOLOGY |
| リソース情報 | |
| ゼブラフィッシュ | ef1a:loxP-GFP-loxP-dsRed2 transgenic line? |