RRC ID |
12176
|
著者 |
Kobayashi N, Fujiwara T, Westerman KA, Inoue Y, Sakaguchi M, Noguchi H, Miyazaki M, Cai J, Tanaka N, Fox IJ, Leboulch P.
|
タイトル |
Prevention of acute liver failure in rats with reversibly immortalized human hepatocytes.
|
ジャーナル |
Science
|
Abstract |
Because of a critical shortage in suitable organs, many patients with terminal liver disease die each year before liver transplantation can be performed. Transplantation of isolated hepatocytes has been proposed for the temporary metabolic support of patients awaiting liver transplantation or spontaneous reversion of their liver disease. A major limitation of this form of therapy is the present inability to isolate an adequate number of transplantable hepatocytes. A highly differentiated cell line, NKNT-3, was generated by retroviral transfer in normal primary adult human hepatocytes of an immortalizing gene that can be subsequently and completely excised by Cre/Lox site-specific recombination. When transplanted into the spleen of rats under transient immunosuppression, reversibly immortalized NKNT-3 cells provided life-saving metabolic support during acute liver failure induced by 90% hepatectomy.
|
巻・号 |
287(5456)
|
ページ |
1258-62
|
公開日 |
2000-2-18
|
DOI |
10.1126/science.287.5456.1258
|
PII |
8282
|
PMID |
10678831
|
MeSH |
Adult
Animals
Antigens, Polyomavirus Transforming / genetics
Cell Culture Techniques / methods*
Cell Differentiation
Cell Line
Cell Transplantation*
Gene Expression
Genetic Vectors
Hepatectomy
Humans
Integrases / metabolism
Liver / cytology*
Liver / metabolism
Liver / pathology
Liver Failure, Acute / metabolism
Liver Failure, Acute / pathology
Liver Failure, Acute / prevention & control*
Liver Failure, Acute / therapy
Liver Regeneration
Mice
Mice, SCID
Rats
Retroviridae / genetics
Spleen / cytology
Transfection
Viral Proteins*
|
IF |
41.846
|
引用数 |
241
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
遺伝子材料 |
AxCANCre (RDB01748) AxCALacZ Adex1CAlacZ (RDB02726) |