| RRC ID |
12349
|
| 著者 |
Yonekura-Sakakibara K, Tohge T, Matsuda F, Nakabayashi R, Takayama H, Niida R, Watanabe-Takahashi A, Inoue E, Saito K.
|
| タイトル |
Comprehensive flavonol profiling and transcriptome coexpression analysis leading to decoding gene-metabolite correlations in Arabidopsis.
|
| ジャーナル |
Plant Cell
|
| Abstract |
To complete the metabolic map for an entire class of compounds, it is essential to identify gene-metabolite correlations of a metabolic pathway. We used liquid chromatography-mass spectrometry (LC-MS) to identify the flavonoids produced by Arabidopsis thaliana wild-type and flavonoid biosynthetic mutant lines. The structures of 15 newly identified and eight known flavonols were deduced by LC-MS profiling of these mutants. Candidate genes presumably involved in the flavonoid pathway were delimited by transcriptome coexpression network analysis using public databases, leading to the detailed analysis of two flavonoid pathway genes, UGT78D3 (At5g17030) and RHM1 (At1g78570). The levels of flavonol 3-O-arabinosides were reduced in ugt78d3 knockdown mutants, suggesting that UGT78D3 is a flavonol arabinosyltransferase. Recombinant UGT78D3 protein could convert quercetin to quercetin 3-O-arabinoside. The strict substrate specificity of UGT78D3 for flavonol aglycones and UDP-arabinose indicate that UGT78D3 is a flavonol arabinosyltransferase. A comparison of flavonol profile in RHM knockout mutants indicated that RHM1 plays a major role in supplying UDP-rhamnose for flavonol modification. The rate of flavonol 3-O-glycosylation is more affected than those of 7-O-glycosylation by the supply of UDP-rhamnose. The precise identification of flavonoids in conjunction with transcriptomics thus led to the identification of a gene function and a more complete understanding of a plant metabolic network.
|
| 巻・号 |
20(8)
|
| ページ |
2160-76
|
| 公開日 |
2008-8-1
|
| DOI |
10.1105/tpc.108.058040
|
| PII |
tpc.108.058040
|
| PMID |
18757557
|
| PMC |
PMC2553606
|
| MeSH |
Arabidopsis / genetics*
Arabidopsis / metabolism
Arabidopsis Proteins / genetics*
Arabidopsis Proteins / metabolism
Chromatography, Liquid
Flavonols / chemistry
Flavonols / metabolism*
Gene Expression Profiling / methods*
Glucosyltransferases / genetics
Glucosyltransferases / metabolism
Molecular Structure
Mutation
Pentosyltransferases / genetics
Pentosyltransferases / metabolism
Plants, Genetically Modified / genetics
Plants, Genetically Modified / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Spectrometry, Mass, Electrospray Ionization
Substrate Specificity
|
| IF |
9.618
|
| 引用数 |
259
|
|
WOS 分野
|
PLANT SCIENCES
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
pda08967
pdx21428
pda04986
pda08987
pda01903
pda07289
pda08132
pda02851
pda00589
pda04519
pda08705 |
