論文 - 詳細
| RRC ID | 12445 |
|---|---|
| 著者 | Teratani T, Domoto T, Kuriki K, Kageyama T, Takayama T, Ishikawa A, Ozono S, Nozawa R. |
| タイトル | Detection of transcript for brain-type fatty Acid-binding protein in tumor and urine of patients with renal cell carcinoma. |
| ジャーナル | Urology |
| Abstract |
OBJECTIVES:To clarify the gene expression patterns of fatty acid-binding protein (FABP) and evaluate it as a potential marker for the diagnosis of renal cell carcinoma (RCC). RCC is the most common renal neoplasm. METHODS:The expression of eight FABP genes in normal human tissues, tumor cell lines, and surgically resected RCC tissues (n = 54) was evaluated by reverse transcriptase-polymerase chain reaction. Additionally, the gene expression of FABPs in the urine of healthy volunteers (n = 12) and patients with RCC (n = 5) was investigated. RESULTS:In these results, the carcinoma tissues but not the noncancerous (normal) parts of the kidney samples resected from patients with RCC expressed the transcript for brain-type FABP (B-FABP), indicating that expression of the B-FABP gene is a novel marker for RCC. Furthermore, the B-FABP cDNA fragment was not amplified by reverse transcriptase-polymerase chain reaction in the urine samples of healthy donors or patients with RCC after surgical operation. However, B-FABP cDNA was amplified in the patients' urine samples collected before surgery. CONCLUSIONS:This novel method can be used as a powerful ancillary in the diagnosis of RCC. |
| 巻・号 | 69(2) |
| ページ | 236-40 |
| 公開日 | 2007-2-1 |
| DOI | 10.1016/j.urology.2006.09.060 |
| PII | S0090-4295(06)02330-2 |
| PMID | 17320655 |
| MeSH | Base Sequence Biomarkers, Tumor / blood* Carcinoma, Renal Cell / genetics* Carcinoma, Renal Cell / pathology Carcinoma, Renal Cell / urine Cell Line, Tumor Chi-Square Distribution DNA, Complementary / analysis Fatty Acid-Binding Proteins / genetics Fatty Acid-Binding Proteins / metabolism* Gene Expression Regulation, Neoplastic Humans Kidney Neoplasms / genetics* Kidney Neoplasms / pathology Kidney Neoplasms / urine Molecular Sequence Data Probability Prognosis RNA / analysis Reverse Transcriptase Polymerase Chain Reaction Sampling Studies Sensitivity and Specificity Tissue Culture Techniques |
| IF | 1.924 |
| 引用数 | 28 |
| WOS 分野 | UROLOGY & NEPHROLOGY |
| リソース情報 | |
| ヒト・動物細胞 | TUHR14TKB(RCB1383) |