| RRC ID |
12595
|
| 著者 |
Onishi M, Iida M, Koga T, Yamada S, Hirata A, Iwaki T, Takegawa K, Fukui Y, Tachikawa H.
|
| タイトル |
Schizosaccharomyces pombe Sst4p, a conserved Vps27/Hrs homolog, functions downstream of phosphatidylinositol 3-kinase Pik3p to mediate proper spore formation.
|
| ジャーナル |
Eukaryot Cell
|
| Abstract |
Sporulation of the fission yeast Schizosaccharomyces pombe is a developmental process that generates gametes and that includes the formation of spore envelope precursors called the forespore membranes. Assembly and development of forespore membranes require vesicular trafficking from other intracellular membrane compartments. We have shown that phosphatidylinositol 3-kinase (PtdIns 3-kinase) is required for efficient and proper development of forespore membranes. The role of a FYVE domain protein, Sst4p, a homolog of Vps27p/Hrs, as a downstream factor for PtdIns 3-kinase in sporulation was investigated. sst4Delta asci formed spores with oval-shaped morphology and with reduced viability compared to that of the wild-type spores. The extension of forespore membranes was inefficient, and bubble-like structures emerged from the leading edges of the forespore membranes. Sst4p localization was examined using fluorescent protein fusions and was found to be adjacent to the forespore membranes during sporulation. The localization and function of Sst4p were dependent on its FYVE domain and on PtdIns 3-kinase. Sst4p colocalized and interacted with Hse1p, a homolog of Saccharomyces cerevisiae Hse1p and of mammalian STAM. Mutations in all three UIM domains of the Sst4p/Hse1p complex resulted in formation of spores with abnormal morphology. These results suggest that Sst4p is a downstream factor of PtdIns 3-kinase and functions in forespore membrane formation.
|
| 巻・号 |
6(12)
|
| ページ |
2343-53
|
| 公開日 |
2007-12-1
|
| DOI |
10.1128/EC.00211-07
|
| PII |
EC.00211-07
|
| PMID |
17951524
|
| PMC |
PMC2168254
|
| MeSH |
Alleles
Amino Acid Sequence
Endosomal Sorting Complexes Required for Transport
Gene Expression Regulation, Fungal*
Microscopy, Electron
Microscopy, Fluorescence
Models, Biological
Molecular Sequence Data
Mutation
Phosphatidylinositol 3-Kinases / metabolism*
Phosphatidylinositol 3-Kinases / physiology
Protein Structure, Tertiary
Saccharomyces cerevisiae / metabolism
Saccharomyces cerevisiae Proteins / metabolism
Saccharomyces cerevisiae Proteins / physiology*
Schizosaccharomyces / genetics
Schizosaccharomyces / metabolism*
Schizosaccharomyces pombe Proteins / metabolism*
Schizosaccharomyces pombe Proteins / physiology
Spores, Fungal / metabolism*
Vesicular Transport Proteins / metabolism
Vesicular Transport Proteins / physiology*
|
| IF |
2.992
|
| 引用数 |
5
|
|
WOS 分野
|
MYCOLOGY
MICROBIOLOGY
|
| リソース情報 |
| 酵母 |
|
