論文 - 詳細
| RRC ID | 12667 |
|---|---|
| 著者 | Kashiwazaki N, Seita Y, Takizawa A, Maedomari N, Ito J, Serikawa T. |
| タイトル | Techniques for in vitro and in vivo fertilization in the rat. |
| ジャーナル | Methods Mol Biol |
| Abstract |
Although in vitro and in vivo fertilization are powerful tools for restoring conserved sperm as well as stocked males in the rat, the techniques have progressively gained importance. However, the techniques are not used extensively for efficient production of rat offspring, because the techniques require a great deal of skill. This chapter describes the protocols for in vitro and in vivo fertilization in the rat. Namely, sperm collection, sperm cryopreservation, pre-incubation of sperm, and insemination (co-culture with sperm and oocytes) for in vitro fertilization and intrauterine insemination for in vivo fertilization with fresh or frozen/thawed spermatozoa are provided. |
| 巻・号 | 597 |
| ページ | 311-22 |
| 公開日 | 2010-1-1 |
| DOI | 10.1007/978-1-60327-389-3_22 |
| PMID | 20013243 |
| MeSH | Animals Coculture Techniques / methods Cryopreservation / methods Cryopreservation / veterinary Female Fertilization* Fertilization in Vitro / methods* Fertilization in Vitro / veterinary* Insemination, Artificial / methods* Insemination, Artificial / veterinary* Male Oocytes / cytology Rats / physiology* Rats, Wistar / physiology Semen Preservation / methods Semen Preservation / veterinary Spermatozoa / cytology |
| 引用数 | 3 |
| WOS 分野 | BIOCHEMICAL RESEARCH METHODS PHYSIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ラット | Technical Report |