| RRC ID |
1282
|
| 著者 |
Matsushima-Hibiya Y, Watanabe M, Hidari KI, Miyamoto D, Suzuki Y, Kasama T, Kasama T, Koyama K, Sugimura T, Wakabayashi K.
|
| タイトル |
Identification of glycosphingolipid receptors for pierisin-1, a guanine-specific ADP-ribosylating toxin from the cabbage butterfly.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
Pierisin-1, a cytotoxic protein found naturally in the cabbage butterfly, induces apoptosis of mammalian cells. Our recent studies suggest that pierisin-1 consists of an N-terminal ADP-ribosyltransferase domain, and a C-terminal region that binds to receptors on the surfaces of target cells and incorporates the protein into cells. The present study was undertaken to identify receptors for pierisin-1. The cross-linking and cloning experiments suggested that the proteins on cell membrane had no binding ability to pierisin-1. Inhibitory assays of fractionated lipids from human cervical carcinoma HeLa cells, which are highly sensitive to pierisin-1, indicated neutral glycosphingolipids on the cell surface to show receptor activity. Inhibitory assays and TLC immunostaining using anti-pierisin-1 antibodies demonstrated two neutral glycosphingolipids as active components. Analysis of their structures with glycosphingolipid-specific antibodies and negative secondary ion mass spectrometry identified them as globotriaosylceramide (Gb3) and globotetraosylceramide (Gb4). The receptor activities of Gb3 and Gb4 for pierisin-1 were also confirmed with these authentic compounds. Pierisin-1-insensitive mouse melanoma MEB4 cells were found to lack pierisin-1 receptors, including Gb3 and Gb4, but pretreatment of the cells with glycosphingolipid Gb3 or Gb4 enhanced their sensitivity to pierisin-1. Thus, Gb3 and Gb4 were proven to serve as pierisin-1 receptors. The C-terminal region of pierisin-1 consists of possible lectin domains of a ricin B-chain, containing QXW sequences, which are essential for its structural organization. Alteration of QXW by site-directed mutagenesis caused marked reduction of pierisin-1 cytotoxicity. Thus, our results suggest that pierisin-1 binds to Gb3 and Gb4 receptors at the C-terminal region, in a manner similar to ricin, and then exhibits cytotoxicity after incorporation into the cell.
|
| 巻・号 |
278(11)
|
| ページ |
9972-8
|
| 公開日 |
2003-3-14
|
| DOI |
10.1074/jbc.m212114200
|
| PII |
S0021-9258(19)71393-3
|
| PMID |
12645583
|
| MeSH |
ADP Ribose Transferases
Amino Acid Sequence
Animals
Base Sequence
Butterflies
Chromatography, Thin Layer
Cross-Linking Reagents / pharmacology
Dose-Response Relationship, Drug
Glycosphingolipids / metabolism*
HeLa Cells
Humans
Inhibitory Concentration 50
Insect Proteins / chemistry*
Insect Proteins / metabolism*
Lectins
Mass Spectrometry
Mice
Models, Molecular
Molecular Sequence Data
Mutagenesis, Site-Directed
Phospholipids / metabolism
Protein Binding
Protein Structure, Tertiary
Receptors, Cell Surface / metabolism*
Sequence Homology, Amino Acid
|
| IF |
4.238
|
| 引用数 |
35
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HeLa(RCB0007)
MEB4(RCB1027) |
