RRC ID |
1469
|
著者 |
Sumita C, Yamane M, Matsuda T, Maeda M, Nariai T, Fujio Y, Azuma J.
|
タイトル |
Platelet activating factor induces cytoskeletal reorganization through Rho family pathway in THP-1 macrophages.
|
ジャーナル |
FEBS Lett
|
Abstract |
In the process of atherosclerosis, platelet activating factor (PAF) promotes the infiltration of inflammatory cells into atherosclerotic plaque by modulating their cytoskeleton. Here, we examined whether Rho family proteins are involved in PAF-induced cytoskeletal reorganization in THP-1 macrophages. PAF stimulation rapidly induced cell elongation, accompanied by filopodia formation. The inhibition of Rho family proteins by the overexpression of Rho-GDI attenuated the PAF-mediated morphological changes. Both RhoA and Cdc42 were activated in response to PAF. Inhibition of RhoA or Cdc42 by dominant negative mutants abrogated morphological changes induced by PAF. Collectively, PAF regulates cytoarchitecture through Rho family proteins in macrophages.
|
巻・号 |
579(18)
|
ページ |
4038-42
|
公開日 |
2005-7-18
|
DOI |
10.1016/j.febslet.2005.06.022
|
PII |
S0014-5793(05)00751-9
|
PMID |
16004986
|
MeSH |
Adenoviridae / genetics
Arteriosclerosis / metabolism
Cell Line, Tumor
Cytoskeleton / metabolism*
Gene Transfer Techniques
Guanine Nucleotide Dissociation Inhibitors / metabolism
Guanosine Triphosphate / chemistry
Guanosine Triphosphate / metabolism
Humans
Immunoblotting
Inflammation
Macrophages / metabolism*
Platelet Activating Factor / metabolism*
Pseudopodia / metabolism
Time Factors
Up-Regulation
beta-Galactosidase / metabolism
cdc42 GTP-Binding Protein / metabolism
rac1 GTP-Binding Protein / metabolism
rho GTP-Binding Proteins / metabolism*
rho-Specific Guanine Nucleotide Dissociation Inhibitors
rhoA GTP-Binding Protein / metabolism
|
IF |
3.057
|
引用数 |
10
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
THP-1(RCB1189) |