| RRC ID |
15410
|
| 著者 |
Shimotoyodome A, Koudate T, Kobayashi H, Nakamura J, Tokimitsu I, Hase T, Inoue T, Matsukubo T, Takaesu Y.
|
| タイトル |
Reduction of Streptococcus mutans adherence and dental biofilm formation by surface treatment with phosphorylated polyethylene glycol.
|
| ジャーナル |
Antimicrob Agents Chemother
|
| Abstract |
Initial attachment of the cariogenic Streptococcus mutans onto dental enamel is largely promoted by the adsorption of specific salivary proteins on enamel surface. Some phosphorylated salivary proteins were found to reduce S. mutans adhesion by competitively inhibiting the adsorption of S. mutans-binding salivary glycoproteins to hydroxyapatite (HA). The aim of this study was to develop antiadherence compounds for preventing dental biofilm development. We synthesized phosphorylated polyethylene glycol (PEG) derivatives and examined the possibility of surface pretreatment with them for preventing S. mutans adhesion in vitro and dental biofilm formation in vivo. Pretreatment of the HA surface with methacryloyloxydecyl phosphate (MDP)-PEG prior to saliva incubation hydrophilized the surface and thereby reduced salivary protein adsorption and saliva-promoted bacterial attachment to HA. However, when MDP-PEG was added to the saliva-pretreated HA (S-HA) surface, its inhibitory effect on bacterial binding was completely diminished. S. mutans adhesion onto S-HA was successfully reduced by treatment of the surface with pyrophosphate (PP), which desorbs salivary components from S-HA. Treatment of S-HA surfaces with MDP-PEG plus PP completely inhibited saliva-promoted S. mutans adhesion even when followed by additional saliva treatment. Finally, mouthwash with MDP-PEG plus PP prevented de novo biofilm development after thorough teeth cleaning in humans compared to either water or PP alone. We conclude that MDP-PEG plus PP has the potential for use as an antiadherence agent that prevents dental biofilm development.
|
| 巻・号 |
51(10)
|
| ページ |
3634-41
|
| 公開日 |
2007-10-1
|
| DOI |
10.1128/AAC.00380-07
|
| PII |
AAC.00380-07
|
| PMID |
17646419
|
| PMC |
PMC2043269
|
| MeSH |
Adult
Amino Acids / chemistry
Animals
Bacterial Adhesion / drug effects*
Biofilms / drug effects*
Dental Plaque / microbiology
Drug Design
Durapatite
Histatins / chemistry
Humans
Male
Parotid Gland / drug effects
Parotid Gland / metabolism
Phosphorylation
Polyethylene Glycols / pharmacology*
Rats
Salivary Proteins and Peptides / chemistry
Salivation / drug effects
Salivation / physiology
Streptococcus mutans / drug effects*
Surface Properties
Tooth / microbiology*
|
| IF |
4.904
|
| 引用数 |
25
|
|
WOS 分野
|
PHARMACOLOGY & PHARMACY
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 5175
JCM 5708
JCM 8353
JCM 8525 |
