| RRC ID |
15711
|
| 著者 |
Hatamoto M, Nakai K, Ohashi A, Imachi H.
|
| タイトル |
Sequence-specific bacterial growth inhibition by peptide nucleic acid targeted to the mRNA binding site of 16S rRNA.
|
| ジャーナル |
Appl Microbiol Biotechnol
|
| Abstract |
Peptide nucleic acid (PNA) targeted to the functional domains of 23S rRNA can inhibit translation and cell growth. However, effective inhibition of translation and cell growth using 16S rRNA-targeted PNA has still not been achieved. Here, we report that PNA targeted to the functional site of 16S rRNA could inhibit both gene expression in vitro and bacterial growth in pure culture with sequence specificity. We used 10-mer PNAs conjugated with a cell-penetrating peptide, which targeted the mRNA binding site at the 3' end of 16S rRNA. Using 0.6 microM of the peptide-PNAs, cell-free ss-galactosidase production decreased by 50%, whereas peptide-PNAs with one or two mismatches to the target sequence showed much weaker inhibition effects. To determine the growth inhibition and bactericidal effects of the peptide-PNA conjugate, we performed OD measurement and viable cell counting. We observed dose- and sequence-dependent inhibition of cell growth and bactericidal effects. These growth inhibitory effects are observed both in the Gram-negative bacterium of Escherichia coli and the Gram-positive bacteria Bacillus subtilis and Corynebacterium efficiens, although inhibitory concentrations were different for each bacterial species. These results present possibilities for 16S rRNA sequence-based specific bacterial growth inhibition using a peptide-PNA conjugate.
|
| 巻・号 |
84(6)
|
| ページ |
1161-8
|
| 公開日 |
2009-10-1
|
| DOI |
10.1007/s00253-009-2099-0
|
| PMID |
19578844
|
| MeSH |
Bacillus subtilis / drug effects
Bacillus subtilis / growth & development
Bacillus subtilis / metabolism
Base Sequence
Binding Sites / genetics
Corynebacterium / drug effects
Corynebacterium / growth & development
Corynebacterium / metabolism
Cupriavidus necator / drug effects
Cupriavidus necator / growth & development
Cupriavidus necator / metabolism
Dose-Response Relationship, Drug
Escherichia coli / drug effects
Escherichia coli / genetics
Escherichia coli / growth & development*
Escherichia coli Infections / drug therapy
Escherichia coli Infections / metabolism
Humans
Peptide Nucleic Acids / genetics
Peptide Nucleic Acids / metabolism*
Peptide Nucleic Acids / therapeutic use
Protein Biosynthesis / drug effects
RNA, Bacterial / genetics
RNA, Bacterial / metabolism
RNA, Messenger / metabolism
RNA, Ribosomal, 16S / chemistry
RNA, Ribosomal, 16S / genetics
RNA, Ribosomal, 16S / metabolism*
|
| IF |
3.53
|
| 引用数 |
23
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 11189 |
