| RRC ID |
15771
|
| 著者 |
Ikai H, Nakamura K, Shirato M, Kanno T, Iwasawa A, Sasaki K, Niwano Y, Kohno M.
|
| タイトル |
Photolysis of hydrogen peroxide, an effective disinfection system via hydroxyl radical formation.
|
| ジャーナル |
Antimicrob Agents Chemother
|
| Abstract |
The relationship between the amount of hydroxyl radicals generated by photolysis of H(2)O(2) and bactericidal activity was examined. H(2)O(2) (1 M) was irradiated with laser light at a wavelength of 405 nm to generate hydroxyl radicals. Electron spin resonance spin trapping analysis showed that the amount of hydroxyl radicals produced increased with the irradiation time. Four species of pathogenic oral bacteria, Staphylococcus aureus, Aggregatibacter actinomycetemcomitans, Streptococcus mutans, and Enterococcus faecalis, were used in the bactericidal assay. S. mutans in a model biofilm was also examined. Laser irradiation of suspensions in 1 M H(2)O(2) resulted in a >99.99% reduction of the viable counts of each of the test species within 3 min of treatment. Treatment of S. mutans in a biofilm resulted in a >99.999% reduction of viable counts within 3 min. Other results demonstrated that the bactericidal activity was dependent on the amount of hydroxyl radicals generated. Treatment of bacteria with 200 to 300 μM hydroxyl radicals would result in reductions of viable counts of >99.99%.
|
| 巻・号 |
54(12)
|
| ページ |
5086-91
|
| 公開日 |
2010-12-1
|
| DOI |
10.1128/AAC.00751-10
|
| PII |
AAC.00751-10
|
| PMID |
20921319
|
| PMC |
PMC2981275
|
| MeSH |
Disinfection / methods*
Electron Spin Resonance Spectroscopy
Enterococcus faecalis / drug effects
Hydrogen Peroxide / chemistry*
Hydroxyl Radical / chemistry*
Hydroxyl Radical / pharmacology
Microbial Sensitivity Tests
Photolysis*
Staphylococcus aureus / drug effects
Streptococcus mutans / drug effects
|
| IF |
4.904
|
| 引用数 |
79
|
|
WOS 分野
|
PHARMACOLOGY & PHARMACY
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 2434
JCM 5705 |
