| RRC ID |
17436
|
| 著者 |
Nariya H, Miyata S, Tamai E, Sekiya H, Maki J, Okabe A.
|
| タイトル |
Identification and characterization of a putative endolysin encoded by episomal phage phiSM101 of Clostridium perfringens.
|
| ジャーナル |
Appl Microbiol Biotechnol
|
| Abstract |
Clostridium perfringens produces potent toxins and histolytic enzymes, causing various diseases including life-threatening fulminant diseases in humans and other animals. Aiming at utilizing a phage endolysin as a therapeutic alternative to antibiotics, we surveyed the genome and bacteriophage sequences of C. perfringens. A phiSM101 muramidase gene (psm) revealed by this study can be assumed to encode an N-acetylmuramidase, since the N-terminal catalytic domain deduced from the gene shows high homology of those of N-acetylmuramidases. The psm gene is characteristic in that it is present in phiSM101, an episomal phage of enterotoxigenic C. perfringens type A strain, SM101, and also in that homologous genes are present in the genomes of all five C. perfringens toxin types. The psm gene was cloned and expressed in Escherichia coli as a protein histidine-tagged at the N-terminus (Psm-his). Psm-his was purified to homogeneity by nickel-charged immobilized metal affinity chromatography and anion-exchange chromatography. The purified enzyme lysed cells of all C. perfringens toxin types but not other clostridial species tested, as was shown by a turbidity reduction assay. These results indicate the Psm-his is useful as a cell-wall lytic enzyme and also suggest that it is potentially useful for biocontrol of this organism.
|
| 巻・号 |
90(6)
|
| ページ |
1973-9
|
| 公開日 |
2011-6-1
|
| DOI |
10.1007/s00253-011-3253-z
|
| PMID |
21484204
|
| MeSH |
Bacteriolysis*
Bacteriophages / enzymology*
Bacteriophages / isolation & purification
Chromatography, Affinity / methods
Cloning, Molecular
Clostridium perfringens / virology*
Endopeptidases / genetics
Endopeptidases / isolation & purification
Endopeptidases / metabolism*
Escherichia coli / genetics
Gene Expression
Glycoside Hydrolases / genetics
Glycoside Hydrolases / isolation & purification
Glycoside Hydrolases / metabolism*
Recombinant Proteins / genetics
Recombinant Proteins / isolation & purification
Recombinant Proteins / metabolism
|
| IF |
3.53
|
| 引用数 |
24
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 1296
JCM 1403
JCM 1419 |
